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6qv7
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6qv7 is ON HOLD until Paper Publication Authors: Lorenzo-Orts, L., Hothorn, M. Description: Crystal structure of a CHAD domain from Chlorobium tepi...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a CHAD domain from Chlorobium tepidum== | |
| + | <StructureSection load='6qv7' size='340' side='right'caption='[[6qv7]], [[Resolution|resolution]] 1.72Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6qv7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlorobaculum_tepidum_TLS Chlorobaculum tepidum TLS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QV7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qv7 OCA], [https://pdbe.org/6qv7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qv7 RCSB], [https://www.ebi.ac.uk/pdbsum/6qv7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qv7 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8KE09_CHLTE Q8KE09_CHLTE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Inorganic polyphosphates (polyPs) are linear polymers of orthophosphate units linked by phosphoanhydride bonds. Here, we report that bacterial, archaeal, and eukaryotic conserved histidine alpha-helical (CHAD) domains are specific polyP-binding modules. Crystal structures reveal that CHAD domains are formed by two four-helix bundles, giving rise to a central pore surrounded by conserved basic surface patches. Different CHAD domains bind polyPs with dissociation constants ranging from the nano- to mid-micromolar range, but not nucleic acids. A CHAD-polyP complex structure reveals the phosphate polymer binding across the central pore and along the two basic patches. Mutational analysis of CHAD-polyP interface residues validates the complex structure. The presence of a CHAD domain in the polyPase ygiF enhances its enzymatic activity. The only known CHAD protein from the plant Ricinus communis localizes to the nucleus/nucleolus when expressed in Arabidopsis and tobacco, suggesting that plants may harbor polyPs in these compartments. We propose that CHAD domains may be used to engineer the properties of polyP-metabolizing enzymes and to specifically localize polyP stores in eukaryotic cells and tissues. | ||
| - | + | Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules.,Lorenzo-Orts L, Hohmann U, Zhu J, Hothorn M Life Sci Alliance. 2019 May 27;2(3). pii: 2/3/e201900385. doi:, 10.26508/lsa.201900385. Print 2019 Jun. PMID:31133615<ref>PMID:31133615</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6qv7" style="background-color:#fffaf0;"></div> |
| - | [[Category: Hothorn | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Chlorobaculum tepidum TLS]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hothorn M]] | ||
| + | [[Category: Lorenzo-Orts L]] | ||
Current revision
Crystal structure of a CHAD domain from Chlorobium tepidum
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