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6agy

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Aspergillus fumigatus Af293 NDK

Structural highlights

6agy is a 1 chain structure with sequence from Aspergillus fumigatus Af293. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NDK_ASPFU Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.

Publication Abstract from PubMed

Aspergillus fumigatus is a major pathogen of invasive pulmonary aspergillosis with high mortality rate. The nucleoside diphosphate kinase of A. fumigatus, AfNDK (also called SwoH) is essential for its viability, however, its structural characteristic was unknown. In this study, we solved the crystal structure of AfNDK and found that it exists predominantly in form of tetramer in solution. Oligomeric form rather than dimeric form was essential for its kinase activity. The Arg30 and the C terminal amino acids were crucial for dimer-dimer interaction and the viability of A. fumigatus. Mutation V83F might make the secondary structure alpha5 helix protrude outward so that the whole protein structure became unstable at higher temperature, which might subsequently result to the inviability of A. fumigatus under 44 degrees C. In conclusion, the crystal structure of AfNDK was for the first time analyzed and the stability of the tetrameric form with dimer-dimer interaction were crucial for its function in A. fumigatus.

Characterization of crystal structure and key residues of Aspergillus fumigatus nucleoside diphosphate kinase.,Hu Y, Jia X, Lu Z, Han L Biochem Biophys Res Commun. 2019 Mar 26;511(1):148-153. doi:, 10.1016/j.bbrc.2019.01.126. Epub 2019 Feb 14. PMID:30773256^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hu Y, Jia X, Lu Z, Han L. Characterization of crystal structure and key residues of Aspergillus fumigatus nucleoside diphosphate kinase. Biochem Biophys Res Commun. 2019 Mar 26;511(1):148-153. doi:, 10.1016/j.bbrc.2019.01.126. Epub 2019 Feb 14. PMID:30773256 doi:http://dx.doi.org/10.1016/j.bbrc.2019.01.126

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6agy"

Categories: Aspergillus fumigatus Af293 | Large Structures | Han L | Hu Y

@@ Line 3: / Line 3: @@
 <StructureSection load='6agy' size='340' side='right'caption='[[6agy]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6agy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AGY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6agy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AGY FirstGlance]. <br>
-</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] </span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6agy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6agy OCA], [http://pdbe.org/6agy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6agy RCSB], [http://www.ebi.ac.uk/pdbsum/6agy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6agy ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6agy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6agy OCA], [https://pdbe.org/6agy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6agy RCSB], [https://www.ebi.ac.uk/pdbsum/6agy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6agy ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/NDK_ASPFU NDK_ASPFU]] Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
+[https://www.uniprot.org/uniprot/NDK_ASPFU NDK_ASPFU] Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 18: @@
 </div>
 <div class="pdbe-citations 6agy" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Aspergillus fumigatus Af293]]
 [[Category: Large Structures]]
-[[Category: Nucleoside-diphosphate kinase]]
+[[Category: Han L]]
-[[Category: Han, L]]
+[[Category: Hu Y]]
-[[Category: Hu, Y]]
-[[Category: Kinase]]
-[[Category: Transferase]]

6agy

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Current revision

Aspergillus fumigatus Af293 NDK

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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