6fop
From Proteopedia
(Difference between revisions)
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<StructureSection load='6fop' size='340' side='right'caption='[[6fop]], [[Resolution|resolution]] 1.40Å' scene=''> | <StructureSection load='6fop' size='340' side='right'caption='[[6fop]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6fop]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FOP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6fop]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FOP FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cthe_0660 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 Clostridium thermocellum])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fop OCA], [http://pdbe.org/6fop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fop RCSB], [http://www.ebi.ac.uk/pdbsum/6fop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fop ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fop OCA], [http://pdbe.org/6fop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fop RCSB], [http://www.ebi.ac.uk/pdbsum/6fop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fop ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The family 81 glycoside hydrolase (GH81) from Clostridium thermocellum is a beta-1,3-glucanase belonging to cellulosomal complex. The gene encoding GH81 from Clostridium thermocellum (CtLam81A) was cloned and expressed displaying a molecular mass of ~82kDa. CtLam81A showed maximum activity against laminarin (100U/mg), followed by curdlan (65U/mg), at pH7.0 and 75 degrees C. CtLam81A displayed Km, 2.1+/-0.12mg/ml and Vmax, 109+/-1.8U/mg, against laminarin under optimized conditions. CtLam81A activity was significantly enhanced by Ca(2+) or Mg(2+) ions. Melting curve analysis of CtLam81A showed an increase in melting temperature from 91 degrees C to 96 degrees C by Ca(2+) or Mg(2+) ions and decreased to 82 degrees C by EDTA, indicating that Ca(2+) and Mg(2+) ions may be involved in catalysis and in maintaining structural integrity. TLC and MALDI-TOF analysis of beta-1,3-glucan hydrolysed products released initially, showed beta-1,3-glucan-oligosaccharides degree of polymerization (DP) from DP2 to DP7, confirming an endo-mode of action. The catalytically inactive mutant CtLam81A-E515A generated by site-directed mutagenesis was co-crystallized and tetragonal crystals diffracting up to 1.4A resolution were obtained. CtLam81A-E515A contained 15 alpha-helices and 38 beta-strands forming a four-domain structure viz. a beta-sandwich domain I at N-terminal, an alpha/beta-domain II, an (alpha/alpha)6 barrel domain III, and a small 5-stranded beta-sandwich domain IV. | ||
| + | |||
| + | Novel insights into the degradation of beta-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolase.,Kumar K, Correia MAS, Pires VMR, Dhillon A, Sharma K, Rajulapati V, Fontes CMGA, Carvalho AL, Goyal A Int J Biol Macromol. 2018 Oct 1;117:890-901. doi: 10.1016/j.ijbiomac.2018.06.003., Epub 2018 Jun 2. PMID:29870811<ref>PMID:29870811</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6fop" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Clostridium thermocellum]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Carvalho, A L]] | [[Category: Carvalho, A L]] | ||
[[Category: Correia, M A.S C]] | [[Category: Correia, M A.S C]] | ||
| - | [[Category: Clostridium thermocellum]] | ||
[[Category: Gh81]] | [[Category: Gh81]] | ||
[[Category: Glucanase]] | [[Category: Glucanase]] | ||
Current revision
Glycoside hydrolase family 81 from Clostridium thermocellum (CtLam81A), Mutant E515A
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