3b60

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==Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form==
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<StructureSection load='3b60' size='340' side='right'caption='[[3b60]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3b60]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B60 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>
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{{STRUCTURE_3b60| PDB=3b60 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b60 OCA], [https://pdbe.org/3b60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b60 RCSB], [https://www.ebi.ac.uk/pdbsum/3b60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b60 ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form'''
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== Function ==
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[https://www.uniprot.org/uniprot/MSBA_SALTY MSBA_SALTY] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. Here we compare four x-ray structures of the bacterial ABC lipid flippase, MsbA, trapped in different conformations, two nucleotide-bound structures and two in the absence of nucleotide. Comparison of the nucleotide-free conformations of MsbA reveals a flexible hinge formed by extracellular loops 2 and 3. This hinge allows the nucleotide-binding domains to disassociate while the ATP-binding half sites remain facing each other. The binding of the nucleotide causes a packing rearrangement of the transmembrane helices and changes the accessibility of the transporter from cytoplasmic (inward) facing to extracellular (outward) facing. The inward and outward openings are mediated by two different sets of transmembrane helix interactions. Altogether, the conformational changes between these structures suggest that large ranges of motion may be required for substrate transport.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/3b60_consurf.spt"</scriptWhenChecked>
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3B60 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B60 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Flexibility in the ABC transporter MsbA: Alternating access with a twist., Ward A, Reyes CL, Yu J, Roth CB, Chang G, Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):19005-10. Epub 2007 Nov 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18024585 18024585]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b60 ConSurf].
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[[Category: Salmonella typhimurium]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Chang, G.]]
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</StructureSection>
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[[Category: Reyes, C L.]]
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[[Category: Large Structures]]
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[[Category: Roth, C B.]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Ward, A.]]
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[[Category: Chang G]]
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[[Category: Yu, J.]]
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[[Category: Reyes CL]]
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[[Category: Abc transporter]]
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[[Category: Roth CB]]
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[[Category: Atp-binding]]
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[[Category: Ward A]]
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[[Category: Hydrolase]]
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[[Category: Yu J]]
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[[Category: Inner membrane]]
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[[Category: Lipid flippase]]
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[[Category: Lipid transport]]
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[[Category: Membrane]]
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[[Category: Membrane protein]]
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[[Category: Msba]]
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[[Category: Nucleotide-binding]]
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[[Category: Transmembrane]]
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Current revision

Crystal Structure of MsbA from Salmonella typhimurium with AMPPNP, higher resolution form

PDB ID 3b60

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