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| | <StructureSection load='4bjq' size='340' side='right'caption='[[4bjq]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='4bjq' size='340' side='right'caption='[[4bjq]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4bjq]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BJQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4bjq]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BJQ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bjp|4bjp]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidoglycan_glycosyltransferase Peptidoglycan glycosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.129 2.4.1.129] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bjq OCA], [https://pdbe.org/4bjq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bjq RCSB], [https://www.ebi.ac.uk/pdbsum/4bjq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bjq ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bjq OCA], [http://pdbe.org/4bjq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bjq RCSB], [http://www.ebi.ac.uk/pdbsum/4bjq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bjq ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | ==See Also== | | ==See Also== |
| - | *[[Penicillin-binding protein|Penicillin-binding protein]] | + | *[[Penicillin-binding protein 3D structures|Penicillin-binding protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Peptidoglycan glycosyltransferase]]
| + | [[Category: Charlier P]] |
| - | [[Category: Charlier, P]] | + | [[Category: Herman R]] |
| - | [[Category: Herman, R]] | + | [[Category: Joris M]] |
| - | [[Category: Joris, M]] | + | [[Category: Kerff F]] |
| - | [[Category: Kerff, F]] | + | [[Category: Rocaboy M]] |
| - | [[Category: Rocaboy, M]] | + | [[Category: Sauvage E]] |
| - | [[Category: Sauvage, E]] | + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Transpeptidase]]
| + | |
| Structural highlights
Publication Abstract from PubMed
In Escherichia coli, penicillin-binding protein 3 (PBP3), also known as FtsI, is a central component of the divisome, catalyzing cross-linking of the cell wall peptidoglycan during cell division. PBP3 is mainly periplasmic, with a 23 residues cytoplasmic tail and a single transmembrane helix. We have solved the crystal structure of a soluble form of PBP3 (PBP357-577) at 2.5 A revealing the two modules of high molecular weight class B PBPs, a carboxy terminal module exhibiting transpeptidase activity and an amino terminal module of unknown function. To gain additional insight, the PBP3 Val88-Ser165 subdomain (PBP388-165), for which the electron density is poorly defined in the PBP3 crystal, was produced and its structure solved by SAD phasing at 2.1 A. The structure shows a three dimensional domain swapping with a beta-strand of one molecule inserted between two strands of the paired molecule, suggesting a possible role in PBP357-577 dimerization.
Crystal Structure of Penicillin-Binding Protein 3 (PBP3) from Escherichia coli.,Sauvage E, Derouaux A, Fraipont C, Joris M, Herman R, Rocaboy M, Schloesser M, Dumas J, Kerff F, Nguyen-Disteche M, Charlier P PLoS One. 2014 May 29;9(5):e98042. doi: 10.1371/journal.pone.0098042. eCollection, 2014. PMID:24875494[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Sauvage E, Derouaux A, Fraipont C, Joris M, Herman R, Rocaboy M, Schloesser M, Dumas J, Kerff F, Nguyen-Disteche M, Charlier P. Crystal Structure of Penicillin-Binding Protein 3 (PBP3) from Escherichia coli. PLoS One. 2014 May 29;9(5):e98042. doi: 10.1371/journal.pone.0098042. eCollection, 2014. PMID:24875494 doi:http://dx.doi.org/10.1371/journal.pone.0098042
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