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| | <StructureSection load='4bhu' size='340' side='right'caption='[[4bhu]], [[Resolution|resolution]] 1.91Å' scene=''> | | <StructureSection load='4bhu' size='340' side='right'caption='[[4bhu]], [[Resolution|resolution]] 1.91Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4bhu]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._atcc_6051 Bacillus subtilis subsp. subtilis str. atcc 6051]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BHU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4bhu]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_NCIB_3610_=_ATCC_6051_=_DSM_10 Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BHU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BHU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.91Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bhu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bhu OCA], [http://pdbe.org/4bhu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bhu RCSB], [http://www.ebi.ac.uk/pdbsum/4bhu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bhu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bhu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bhu OCA], [https://pdbe.org/4bhu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bhu RCSB], [https://www.ebi.ac.uk/pdbsum/4bhu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bhu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/YUAB_BACSU YUAB_BACSU]] Required for complex colony architecture. | + | [https://www.uniprot.org/uniprot/BSLA_BACSU BSLA_BACSU] Involved in biofilm formation (PubMed:18978066, PubMed:21742882). Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm (PubMed:22571672, PubMed:23904481). The layer is stable and capable of resistance to high mechanical force compression (PubMed:28701036). Required for complex colony architecture (PubMed:18978066). May function synergistically with exopolysaccharides and TasA amyloid fibers to facilitate the assembly of the biofilm matrix (PubMed:21742882).<ref>PMID:18978066</ref> <ref>PMID:21742882</ref> <ref>PMID:22571672</ref> <ref>PMID:23904481</ref> <ref>PMID:28701036</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus subtilis subsp. subtilis str. atcc 6051]] | + | [[Category: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Aalten, D M.F van]]
| + | [[Category: Bromley KM]] |
| - | [[Category: Bromley, K M]] | + | [[Category: Hobley L]] |
| - | [[Category: Hobley, L]] | + | [[Category: MacPhee CE]] |
| - | [[Category: MacPhee, C E]] | + | [[Category: Ostrowski A]] |
| - | [[Category: Ostrowski, A]] | + | [[Category: Porter M]] |
| - | [[Category: Porter, M]] | + | [[Category: Prescott AR]] |
| - | [[Category: Prescott, A R]] | + | [[Category: Rao FV]] |
| - | [[Category: Rao, F V]] | + | [[Category: Stanley-Wall NR]] |
| - | [[Category: Stanley-Wall, N R]] | + | [[Category: Swedlow JR]] |
| - | [[Category: Swedlow, J R]] | + | [[Category: Van Aalten DMF]] |
| - | [[Category: Biofilm]] | + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Function
BSLA_BACSU Involved in biofilm formation (PubMed:18978066, PubMed:21742882). Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm (PubMed:22571672, PubMed:23904481). The layer is stable and capable of resistance to high mechanical force compression (PubMed:28701036). Required for complex colony architecture (PubMed:18978066). May function synergistically with exopolysaccharides and TasA amyloid fibers to facilitate the assembly of the biofilm matrix (PubMed:21742882).[1] [2] [3] [4] [5]
Publication Abstract from PubMed
Biofilms represent the predominant mode of microbial growth in the natural environment. Bacillus subtilis is a ubiquitous Gram-positive soil bacterium that functions as an effective plant growth-promoting agent. The biofilm matrix is composed of an exopolysaccharide and an amyloid fiber-forming protein, TasA, and assembles with the aid of a small secreted protein, BslA. Here we show that natively synthesized and secreted BslA forms surface layers around the biofilm. Biophysical analysis demonstrates that BslA can self-assemble at interfaces, forming an elastic film. Molecular function is revealed from analysis of the crystal structure of BslA, which consists of an Ig-type fold with the addition of an unusual, extremely hydrophobic "cap" region. A combination of in vivo biofilm formation and in vitro biophysical analysis demonstrates that the central hydrophobic residues of the cap are essential to allow a hydrophobic, nonwetting biofilm to form as they control the surface activity of the BslA protein. The hydrophobic cap exhibits physiochemical properties remarkably similar to the hydrophobic surface found in fungal hydrophobins; thus, BslA is a structurally defined bacterial hydrophobin. We suggest that biofilms formed by other species of bacteria may have evolved similar mechanisms to provide protection to the resident bacterial community.
BslA is a self-assembling bacterial hydrophobin that coats the Bacillus subtilis biofilm.,Hobley L, Ostrowski A, Rao FV, Bromley KM, Porter M, Prescott AR, Macphee CE, van Aalten DM, Stanley-Wall NR Proc Natl Acad Sci U S A. 2013 Jul 31. PMID:23904481[6]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Verhamme DT, Murray EJ, Stanley-Wall NR. DegU and Spo0A jointly control transcription of two loci required for complex colony development by Bacillus subtilis. J Bacteriol. 2009 Jan;191(1):100-8. PMID:18978066 doi:10.1128/JB.01236-08
- ↑ Ostrowski A, Mehert A, Prescott A, Kiley TB, Stanley-Wall NR. YuaB functions synergistically with the exopolysaccharide and TasA amyloid fibers to allow biofilm formation by Bacillus subtilis. J Bacteriol. 2011 Sep;193(18):4821-31. PMID:21742882 doi:10.1128/JB.00223-11
- ↑ Kobayashi K, Iwano M. BslA(YuaB) forms a hydrophobic layer on the surface of Bacillus subtilis biofilms. Mol Microbiol. 2012 Jul;85(1):51-66. PMID:22571672 doi:10.1111/j.1365-2958.2012.08094.x
- ↑ Hobley L, Ostrowski A, Rao FV, Bromley KM, Porter M, Prescott AR, Macphee CE, van Aalten DM, Stanley-Wall NR. BslA is a self-assembling bacterial hydrophobin that coats the Bacillus subtilis biofilm. Proc Natl Acad Sci U S A. 2013 Jul 31. PMID:23904481 doi:10.1073/pnas.1306390110
- ↑ Liu W, Li S, Wang Z, Yan ECY, Leblanc RM. Characterization of Surface-Active Biofilm Protein BslA in Self-Assembling Langmuir Monolayer at the Air-Water Interface. Langmuir. 2017 Aug 1;33(30):7548-7555. PMID:28701036 doi:10.1021/acs.langmuir.7b01739
- ↑ Hobley L, Ostrowski A, Rao FV, Bromley KM, Porter M, Prescott AR, Macphee CE, van Aalten DM, Stanley-Wall NR. BslA is a self-assembling bacterial hydrophobin that coats the Bacillus subtilis biofilm. Proc Natl Acad Sci U S A. 2013 Jul 31. PMID:23904481 doi:10.1073/pnas.1306390110
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