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| | <StructureSection load='4bj4' size='340' side='right'caption='[[4bj4]], [[Resolution|resolution]] 1.72Å' scene=''> | | <StructureSection load='4bj4' size='340' side='right'caption='[[4bj4]], [[Resolution|resolution]] 1.72Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4bj4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BJ4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4bj4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BJ4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.722Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bol|4bol]], [[4bpa|4bpa]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bj4 OCA], [https://pdbe.org/4bj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bj4 RCSB], [https://www.ebi.ac.uk/pdbsum/4bj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bj4 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bj4 OCA], [http://pdbe.org/4bj4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bj4 RCSB], [http://www.ebi.ac.uk/pdbsum/4bj4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bj4 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9HT86_PSEAE Q9HT86_PSEAE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: N-acetylmuramoyl-L-alanine amidase]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Pseae]]
| + | [[Category: Artola-Recolons C]] |
| - | [[Category: Artola-Recolons, C]] | + | [[Category: Carrasco-Lopez C]] |
| - | [[Category: Carrasco-Lopez, C]] | + | [[Category: Hermoso JA]] |
| - | [[Category: Hermoso, J A]] | + | [[Category: Martinez-Caballero CS]] |
| - | [[Category: Martinez-Caballero, C S]] | + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Periplasmic amidase]]
| + | |
| Structural highlights
Function
Q9HT86_PSEAE
Publication Abstract from PubMed
The zinc protease AmpDh2 is a virulence determinant of Pseudomonas aeruginosa , a problematic human pathogen. The mechanism of how the protease manifests virulence is not known, but it is known that it turns over the bacterial cell wall. The reaction of AmpDh2 with the cell wall was investigated, and nine distinct turnover products were characterized by LC/MS/MS. The enzyme turns over both the cross-linked and noncross-linked cell wall. Three high-resolution X-ray structures, the apo enzyme and two complexes with turnover products, were solved. The X-ray structures show how the dimeric protein interacts with the inner leaflet of the bacterial outer membrane and that the two monomers provide a more expansive surface for recognition of the cell wall. This binding surface can accommodate the 3D solution structure of the cross-linked cell wall.
Reaction Products and the X-ray Structure of AmpDh2, a Virulence Determinant of Pseudomonas aeruginosa.,Martinez-Caballero S, Lee M, Artola-Recolons C, Carrasco-Lopez C, Hesek D, Spink E, Lastochkin E, Zhang W, Hellman LM, Boggess B, Mobashery S, Hermoso JA J Am Chem Soc. 2013 Jul 8. PMID:23819763[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Martinez-Caballero S, Lee M, Artola-Recolons C, Carrasco-Lopez C, Hesek D, Spink E, Lastochkin E, Zhang W, Hellman LM, Boggess B, Mobashery S, Hermoso JA. Reaction Products and the X-ray Structure of AmpDh2, a Virulence Determinant of Pseudomonas aeruginosa. J Am Chem Soc. 2013 Jul 8. PMID:23819763 doi:10.1021/ja405464b
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