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| | <StructureSection load='4boc' size='340' side='right'caption='[[4boc]], [[Resolution|resolution]] 2.65Å' scene=''> | | <StructureSection load='4boc' size='340' side='right'caption='[[4boc]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4boc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BOC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BOC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4boc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BOC FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4boc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4boc OCA], [http://pdbe.org/4boc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4boc RCSB], [http://www.ebi.ac.uk/pdbsum/4boc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4boc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4boc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4boc OCA], [https://pdbe.org/4boc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4boc RCSB], [https://www.ebi.ac.uk/pdbsum/4boc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4boc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RPOM_HUMAN RPOM_HUMAN]] DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.<ref>PMID:21278163</ref> | + | [https://www.uniprot.org/uniprot/RPOM_HUMAN RPOM_HUMAN] DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.<ref>PMID:21278163</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[RNA polymerase|RNA polymerase]] | + | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: DNA-directed RNA polymerase]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Human]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Agaronyan, K]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Cheung, A]] | + | [[Category: Agaronyan K]] |
| - | [[Category: Cramer, P]] | + | [[Category: Cheung A]] |
| - | [[Category: Morozov, Y]] | + | [[Category: Cramer P]] |
| - | [[Category: Schwinghammer, K]] | + | [[Category: Morozov Y]] |
| - | [[Category: Temiakov, D]] | + | [[Category: Schwinghammer K]] |
| - | [[Category: Dna-rna hybrid]]
| + | [[Category: Temiakov D]] |
| - | [[Category: Mitochondria]]
| + | |
| - | [[Category: Rna polymerase]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
RPOM_HUMAN DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.[1]
Publication Abstract from PubMed
Here we report the crystal structure of the human mitochondrial RNA polymerase (mtRNAP) transcription elongation complex, determined at 2.65-A resolution. The structure reveals a 9-bp hybrid formed between the DNA template and the RNA transcript and one turn of DNA both upstream and downstream of the hybrid. Comparisons with the distantly related RNA polymerase (RNAP) from bacteriophage T7 indicates conserved mechanisms for substrate binding and nucleotide incorporation but also strong mechanistic differences. Whereas T7 RNAP refolds during the transition from initiation to elongation, mtRNAP adopts an intermediary conformation that is capable of elongation without refolding. The intercalating hairpin that melts DNA during T7 RNAP initiation separates RNA from DNA during mtRNAP elongation. Newly synthesized RNA exits toward the pentatricopeptide repeat (PPR) domain, a unique feature of mtRNAP with conserved RNA-recognition motifs.
Structure of human mitochondrial RNA polymerase elongation complex.,Schwinghammer K, Cheung AC, Morozov YI, Agaronyan K, Temiakov D, Cramer P Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2683. PMID:24096365[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Minczuk M, He J, Duch AM, Ettema TJ, Chlebowski A, Dzionek K, Nijtmans LG, Huynen MA, Holt IJ. TEFM (c17orf42) is necessary for transcription of human mtDNA. Nucleic Acids Res. 2011 May;39(10):4284-99. doi: 10.1093/nar/gkq1224. Epub 2011, Jan 28. PMID:21278163 doi:10.1093/nar/gkq1224
- ↑ Schwinghammer K, Cheung AC, Morozov YI, Agaronyan K, Temiakov D, Cramer P. Structure of human mitochondrial RNA polymerase elongation complex. Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2683. PMID:24096365 doi:http://dx.doi.org/10.1038/nsmb.2683
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