|
|
| (One intermediate revision not shown.) |
| Line 3: |
Line 3: |
| | <StructureSection load='4bq0' size='340' side='right'caption='[[4bq0]], [[Resolution|resolution]] 1.77Å' scene=''> | | <StructureSection load='4bq0' size='340' side='right'caption='[[4bq0]], [[Resolution|resolution]] 1.77Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4bq0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BQ0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4bq0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BQ0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bq0 OCA], [http://pdbe.org/4bq0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bq0 RCSB], [http://www.ebi.ac.uk/pdbsum/4bq0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bq0 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bq0 OCA], [https://pdbe.org/4bq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bq0 RCSB], [https://www.ebi.ac.uk/pdbsum/4bq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bq0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/BAUA_PSEAE BAUA_PSEAE] Involved in the degradation of beta-alanine. Catalyzes the transfer of the amino group from beta-alanine to pyruvate to yield L-alanine and 3-oxopropanoate. It can also accept both 4-aminobutyrate and (S)-alpha-methylbenzylamine (MBA) as amino-group donors in the presence of pyruvate as an amine acceptor.<ref>PMID:21622750</ref> <ref>PMID:23519665</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 24: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pseae]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Isupov, M N]] | + | [[Category: Isupov MN]] |
| - | [[Category: Lebedev, A A]] | + | [[Category: Lebedev AA]] |
| - | [[Category: Littlechild, J A]] | + | [[Category: Littlechild JA]] |
| - | [[Category: Sayer, C]] | + | [[Category: Sayer C]] |
| - | [[Category: Westlake, A]] | + | [[Category: Westlake A]] |
| - | [[Category: Pseudo-translation]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
BAUA_PSEAE Involved in the degradation of beta-alanine. Catalyzes the transfer of the amino group from beta-alanine to pyruvate to yield L-alanine and 3-oxopropanoate. It can also accept both 4-aminobutyrate and (S)-alpha-methylbenzylamine (MBA) as amino-group donors in the presence of pyruvate as an amine acceptor.[1] [2]
Publication Abstract from PubMed
The presence of pseudo-symmetry in a macromolecular crystal and its interplay with twinning may lead to an incorrect space-group (SG) assignment. Moreover, if the pseudo-symmetry is very close to an exact crystallographic symmetry, the structure can be solved and partially refined in the wrong SG. Typically, in such incorrectly determined structures all or some of the pseudo-symmetry operations are, in effect, taken for crystallographic symmetry operations and vice versa. A mistake only becomes apparent when the Rfree ceases to decrease below 0.39 and further model rebuilding and refinement cannot improve the refinement statistics. If pseudo-symmetry includes pseudo-translation, the uncertainty in SG assignment may be associated with an incorrect choice of origin, as demonstrated by the series of examples provided here. The program Zanuda presented in this article was developed for the automation of SG validation. Zanuda runs a series of refinements in SGs compatible with the observed unit-cell parameters and chooses the model with the highest symmetry SG from a subset of models that have the best refinement statistics.
Space-group and origin ambiguity in macromolecular structures with pseudo-symmetry and its treatment with the program Zanuda.,Lebedev AA, Isupov MN Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2430-43. doi:, 10.1107/S1399004714014795. Epub 2014 Aug 29. PMID:25195756[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yao X, He W, Lu CD. Functional characterization of seven γ-Glutamylpolyamine synthetase genes and the bauRABCD locus for polyamine and β-Alanine utilization in Pseudomonas aeruginosa PAO1. J Bacteriol. 2011 Aug;193(15):3923-30. PMID:21622750 doi:10.1128/JB.05105-11
- ↑ Sayer C, Isupov MN, Westlake A, Littlechild JA. Structural studies of Pseudomonas and Chromobacterium omega-aminotransferases provide insights into their differing substrate specificity. Acta Crystallogr D Biol Crystallogr. 2013 Apr;69(Pt 4):564-76. doi:, 10.1107/S0907444912051670. Epub 2013 Mar 14. PMID:23519665 doi:10.1107/S0907444912051670
- ↑ Lebedev AA, Isupov MN. Space-group and origin ambiguity in macromolecular structures with pseudo-symmetry and its treatment with the program Zanuda. Acta Crystallogr D Biol Crystallogr. 2014 Sep 1;70(Pt 9):2430-43. doi:, 10.1107/S1399004714014795. Epub 2014 Aug 29. PMID:25195756 doi:http://dx.doi.org/10.1107/S1399004714014795
|
