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| | <StructureSection load='4bzw' size='340' side='right'caption='[[4bzw]], [[Resolution|resolution]] 2.15Å' scene=''> | | <StructureSection load='4bzw' size='340' side='right'caption='[[4bzw]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4bzw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacpl Lacpl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BZW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4bzw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum_WCFS1 Lactiplantibacillus plantarum WCFS1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BZW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=144:TRIS-HYDROXYMETHYL-METHYL-AMMONIUM'>144</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.148Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=144:TRIS-HYDROXYMETHYL-METHYL-AMMONIUM'>144</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzw OCA], [http://pdbe.org/4bzw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bzw RCSB], [http://www.ebi.ac.uk/pdbsum/4bzw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bzw ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bzw OCA], [https://pdbe.org/4bzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bzw RCSB], [https://www.ebi.ac.uk/pdbsum/4bzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bzw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F9US10_LACPL F9US10_LACPL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Lipase|Lipase]] | + | *[[Lipase 3D Structures|Lipase 3D Structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Carboxylesterase]] | + | [[Category: Lactiplantibacillus plantarum WCFS1]] |
| - | [[Category: Lacpl]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Acebron, I]] | + | [[Category: Acebron I]] |
| - | [[Category: Alvarez, Y]] | + | [[Category: Alvarez Y]] |
| - | [[Category: Benavente, R]] | + | [[Category: Benavente R]] |
| - | [[Category: Esteban-Torres, M]] | + | [[Category: Esteban-Torres M]] |
| - | [[Category: Mancheno, J M]] | + | [[Category: Mancheno JM]] |
| - | [[Category: Munoz, R]] | + | [[Category: Munoz R]] |
| - | [[Category: DelasRivas, B]] | + | [[Category: DelasRivas B]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Lactic acid bacteria]]
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| Structural highlights
Function
F9US10_LACPL
Publication Abstract from PubMed
The alpha/beta hydrolase fold is one of the most versatile structures in the protein realm according to the diversity of sequences adopting such a three dimensional architecture. Here, we report the crystal structure of the carboxylesterase Cest-2923 from the lactic acid bacterium Lactobacillus plantarum WCFS1 refined to 2.1 A resolution, determined its main biochemical characteristics and also carried out an analysis of its associative behaviour in solution. We found that the versatility of a canonical alpha/beta-hydrolase fold, the basic framework of the crystal structure of Cest-2923, also extends to its oligomeric behavior in solution. Thus, we discovered that Cest-2923 exhibits a pH-dependent pleomorphic behaviour in solution involving monomers, canonical dimers and tetramers. Whereas at neutral pH the system is mainly shifted to dimeric species, at acidic conditions tetrameric species predominate. Interestingly, despite that these tetramers result from the association of canonical dimers, as commonly found in many other carboxylesterases from the hormone-sensitive lipase family, they can be defined as "non canonical" since they represent a different association mode. We identified this same type of tetramers in the closest relative of Cest-2923 structurally characterized, the sugar hydrolase YeeB from Lactococcus lactis. Interestingly, the observed associative behaviour is consistent with different crystallographic results of Cest-2923 from structural genomics consortia. Finally, we benefit from the presence of sulphate or acetate molecules (depending on the crystal form analysed) in the close vicinity of the nucleophile Ser116, to identify interactions with the putative oxyanion hole and also to deduce the existence of hydrolytic activity within Cest-2923 crystals. This article is protected by copyright. All rights reserved.
Structure, biochemical characterization and analysis of the pleomorphism of carboxylesterase Cest-2923 from Lactobacillus plantarum WCFS1.,Benavente R, Esteban-Torres M, Acebron I, de Las Rivas B, Munoz R, Alvarez Y, Mancheno JM FEBS J. 2013 Oct 16. doi: 10.1111/febs.12569. PMID:24127688[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Benavente R, Esteban-Torres M, Acebron I, de Las Rivas B, Munoz R, Alvarez Y, Mancheno JM. Structure, biochemical characterization and analysis of the pleomorphism of carboxylesterase Cest-2923 from Lactobacillus plantarum WCFS1. FEBS J. 2013 Oct 16. doi: 10.1111/febs.12569. PMID:24127688 doi:http://dx.doi.org/10.1111/febs.12569
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