2jj7

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[[Image:2jj7.jpg|left|200px]]
 
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==Crystal structure of the HlyIIR mutant protein with residues 170-185 substituted by alanine==
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The line below this paragraph, containing "STRUCTURE_2jj7", creates the "Structure Box" on the page.
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<StructureSection load='2jj7' size='340' side='right'caption='[[2jj7]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jj7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JJ7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jj7 OCA], [https://pdbe.org/2jj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jj7 RCSB], [https://www.ebi.ac.uk/pdbsum/2jj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jj7 ProSAT]</span></td></tr>
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{{STRUCTURE_2jj7| PDB=2jj7 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7X506_BACCE Q7X506_BACCE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jj/2jj7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jj7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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HlyIIR is the negative transcriptional regulator of the hemolysin II gene from Bacillus cereus. Previous X-ray studies showed that HlyIIR contains a disordered loop (a.a. 170-185) located within the C-terminal domain near dimerization interface. To understand the influence of this region on HlyIIR properties and for a potential improvement in the crystallogenesis of the HlyIIR, we constructed a mutant of HlyIIR in which this disordered region is substituted by a single alanine residue. Biochemical analysis of the mutant indicated that it still forms a dimer but the DNA-binding activity is lost. HlyIIR mutant displayed improved crystallization properties and its structure was determined by X-ray crystallography at 2.1 A resolution. Unexpectedly, the structure shows that the HlyIIR mutant forms an alternative dimer with subunits rotated by 160 degrees. Moreover, there are also changes in the conformation of individual subunits. These dramatic structural rearrangements are caused by changes in the conformation of the segment Pro161-Ser169. We conclude that correct conformation of this segment is principal for maintaining the structure and activity of HlyIIR.
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'''CRYSTAL STRUCTURE OF THE HLYIIR MUTANT PROTEIN WITH RESIDUES 170-185 SUBSTITUTED BY ALANINE'''
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[Contraction of the disordered loop located within C-terminal domain of the transcriptional regulator HlyIIR causes its structural rearrangement],Kovalevskii OV, Antson AA, Solonin AS Mol Biol (Mosk). 2009 Jan-Feb;43(1):126-35. PMID:19334535<ref>PMID:19334535</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2JJ7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JJ7 OCA].
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<div class="pdbe-citations 2jj7" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacillus cereus]]
[[Category: Bacillus cereus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Antson, A A.]]
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[[Category: Antson AA]]
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[[Category: Kovalevskiy, O V.]]
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[[Category: Kovalevskiy OV]]
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[[Category: Solonin, A S.]]
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[[Category: Solonin AS]]
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[[Category: Dna-binding]]
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[[Category: Dna-binding protein]]
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[[Category: Tetr family]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Transcriptional regulator]]
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Current revision

Crystal structure of the HlyIIR mutant protein with residues 170-185 substituted by alanine

PDB ID 2jj7

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