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2juo

From Proteopedia

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(New page: 200px <!-- The line below this paragraph, containing "STRUCTURE_2juo", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...)
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[[Image:2juo.jpg|left|200px]]
 
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==GABPa OST domain==
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The line below this paragraph, containing "STRUCTURE_2juo", creates the "Structure Box" on the page.
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<StructureSection load='2juo' size='340' side='right'caption='[[2juo]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2juo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JUO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2juo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2juo OCA], [https://pdbe.org/2juo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2juo RCSB], [https://www.ebi.ac.uk/pdbsum/2juo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2juo ProSAT]</span></td></tr>
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{{STRUCTURE_2juo| PDB=2juo | SCENE= }}
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</table>
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== Function ==
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'''GABPa OST domain'''
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[https://www.uniprot.org/uniprot/GABPA_MOUSE GABPA_MOUSE] Transcription factor capable of interacting with purine rich repeats (GA repeats).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/2juo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2juo ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Using NMR spectroscopy, we identified and characterized a previously unrecognized structured domain near the N-terminus (residues 35-121) of the ETS family transcription factor GABP alpha. The monomeric domain folds as a five-stranded beta-sheet crossed by a distorted helix. Although globally resembling ubiquitin, the GABP alpha fragment differs in its secondary structure topology and thus appears to represent a new protein fold that we term the OST (On-SighT) domain. The surface of the GABP alpha OST domain contains two predominant clusters of negatively-charged residues suggestive of electrostatically driven interactions with positively-charged partner proteins. Following a best-candidate approach to identify such a partner, we demonstrated through NMR-monitored titrations and glutathione S-transferase pulldown assays that the OST domain binds to the CH1 and CH3 domains of the co-activator histone acetyltransferase CBP/p300. This provides a direct structural link between GABP and a central component of the transcriptional machinery.
Using NMR spectroscopy, we identified and characterized a previously unrecognized structured domain near the N-terminus (residues 35-121) of the ETS family transcription factor GABP alpha. The monomeric domain folds as a five-stranded beta-sheet crossed by a distorted helix. Although globally resembling ubiquitin, the GABP alpha fragment differs in its secondary structure topology and thus appears to represent a new protein fold that we term the OST (On-SighT) domain. The surface of the GABP alpha OST domain contains two predominant clusters of negatively-charged residues suggestive of electrostatically driven interactions with positively-charged partner proteins. Following a best-candidate approach to identify such a partner, we demonstrated through NMR-monitored titrations and glutathione S-transferase pulldown assays that the OST domain binds to the CH1 and CH3 domains of the co-activator histone acetyltransferase CBP/p300. This provides a direct structural link between GABP and a central component of the transcriptional machinery.
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==About this Structure==
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Identification and structural characterization of a CBP/p300-binding domain from the ETS family transcription factor GABP alpha.,Kang HS, Nelson ML, Mackereth CD, Scharpf M, Graves BJ, McIntosh LP J Mol Biol. 2008 Mar 28;377(3):636-46. Epub 2008 Jan 30. PMID:18295234<ref>PMID:18295234</ref>
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2JUO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUO OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Identification and structural characterization of a CBP/p300-binding domain from the ETS family transcription factor GABP alpha., Kang HS, Nelson ML, Mackereth CD, Scharpf M, Graves BJ, McIntosh LP, J Mol Biol. 2008 Mar 28;377(3):636-46. Epub 2008 Jan 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18295234 18295234]
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</div>
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<div class="pdbe-citations 2juo" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Graves BJ]]
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[[Category: Graves, B J.]]
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[[Category: Kang H]]
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[[Category: Kang, H.]]
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[[Category: Mackereth CD]]
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[[Category: Mackereth, C D.]]
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[[Category: McIntosh LP]]
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[[Category: McIntosh, L P.]]
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[[Category: Nelson ML]]
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[[Category: Nelson, M L.]]
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[[Category: Schaerpf M]]
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[[Category: Schaerpf, M.]]
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[[Category: Dna-binding]]
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[[Category: Ga-binding protein]]
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[[Category: Nmr ensemble]]
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[[Category: Nucleus]]
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[[Category: Ost]]
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[[Category: Transcription factor]]
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[[Category: Transcription regulation]]
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[[Category: Ubiquitin]]
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Current revision

GABPa OST domain

PDB ID 2juo

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