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| | ==Adeno-Associated Virus 2 in complex with AAVR== | | ==Adeno-Associated Virus 2 in complex with AAVR== |
| - | <StructureSection load='6ihb' size='340' side='right'caption='[[6ihb]], [[Resolution|resolution]] 2.84Å' scene=''> | + | <SX load='6ihb' size='340' side='right' viewer='molstar' caption='[[6ihb]], [[Resolution|resolution]] 2.84Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ihb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Adeno-associated_virus_2_(isolate_srivastava/1982) Adeno-associated virus 2 (isolate srivastava/1982)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IHB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IHB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ihb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Adeno-associated_virus_2_Srivastava/1982 Adeno-associated virus 2 Srivastava/1982] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IHB FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ihb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ihb OCA], [http://pdbe.org/6ihb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ihb RCSB], [http://www.ebi.ac.uk/pdbsum/6ihb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ihb ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.84Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ihb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ihb OCA], [https://pdbe.org/6ihb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ihb RCSB], [https://www.ebi.ac.uk/pdbsum/6ihb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ihb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Disease == | | == Disease == |
| - | [[http://www.uniprot.org/uniprot/K319L_HUMAN K319L_HUMAN]] Systemic lupus erythematosus;Limited cutaneous systemic sclerosis. | + | [https://www.uniprot.org/uniprot/K319L_HUMAN K319L_HUMAN] Systemic lupus erythematosus;Limited cutaneous systemic sclerosis. |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/K319L_HUMAN K319L_HUMAN]] Possible role in axon guidance through interaction with RTN4R.<ref>PMID:20697954</ref> [[http://www.uniprot.org/uniprot/CAPSD_AAV2S CAPSD_AAV2S]] Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus.<ref>PMID:10684294</ref> <ref>PMID:11961250</ref> <ref>PMID:16940508</ref> <ref>PMID:9445046</ref> | + | [https://www.uniprot.org/uniprot/K319L_HUMAN K319L_HUMAN] Possible role in axon guidance through interaction with RTN4R.<ref>PMID:20697954</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6ihb" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6ihb" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| | + | [[Category: Adeno-associated virus 2 Srivastava/1982]] |
| | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lou, Z Y]] | + | [[Category: Lou ZY]] |
| - | [[Category: Zhang, R]] | + | [[Category: Zhang R]] |
| - | [[Category: Aav2]]
| + | |
| - | [[Category: Virus]]
| + | |
| Structural highlights
Disease
K319L_HUMAN Systemic lupus erythematosus;Limited cutaneous systemic sclerosis.
Function
K319L_HUMAN Possible role in axon guidance through interaction with RTN4R.[1]
Publication Abstract from PubMed
Adeno-associated virus (AAV) is a leading vector for virus-based gene therapy. The receptor for AAV (AAVR; also named KIAA0319L) was recently identified, and the precise characterization of AAV-AAVR recognition is in immediate demand. Taking advantage of a particle-filtering algorithm, we report here the cryo-electron microscopy structure of the AAV2-AAVR complex at 2.8 A resolution. This structure reveals that of the five Ig-like polycystic kidney disease (PKD) domains in AAVR, PKD2 binds directly to the spike region of the AAV2 capsid adjacent to the icosahedral three-fold axis. Residues in strands B and E, and the BC loop of AAVR PKD2 interact directly with the AAV2 capsid. The interacting residues in the AAV2 capsid are mainly in AAV-featured variable regions. Mutagenesis of the amino acids at the AAV2-AAVR interface reduces binding activity and viral infectivity. Our findings provide insights into the biology of AAV entry with high-resolution details, providing opportunities for the development of new AAV vectors for gene therapy.
Adeno-associated virus 2 bound to its cellular receptor AAVR.,Zhang R, Cao L, Cui M, Sun Z, Hu M, Zhang R, Stuart W, Zhao X, Yang Z, Li X, Sun Y, Li S, Ding W, Lou Z, Rao Z Nat Microbiol. 2019 Feb 11. pii: 10.1038/s41564-018-0356-7. doi:, 10.1038/s41564-018-0356-7. PMID:30742069[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Poon MW, Tsang WH, Chan SO, Li HM, Ng HK, Waye MM. Dyslexia-associated kiaa0319-like protein interacts with axon guidance receptor nogo receptor 1. Cell Mol Neurobiol. 2011 Jan;31(1):27-35. doi: 10.1007/s10571-010-9549-1. Epub, 2010 Aug 10. PMID:20697954 doi:http://dx.doi.org/10.1007/s10571-010-9549-1
- ↑ Zhang R, Cao L, Cui M, Sun Z, Hu M, Zhang R, Stuart W, Zhao X, Yang Z, Li X, Sun Y, Li S, Ding W, Lou Z, Rao Z. Adeno-associated virus 2 bound to its cellular receptor AAVR. Nat Microbiol. 2019 Feb 11. pii: 10.1038/s41564-018-0356-7. doi:, 10.1038/s41564-018-0356-7. PMID:30742069 doi:http://dx.doi.org/10.1038/s41564-018-0356-7
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