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| | <StructureSection load='4csk' size='340' side='right'caption='[[4csk]], [[Resolution|resolution]] 3.28Å' scene=''> | | <StructureSection load='4csk' size='340' side='right'caption='[[4csk]], [[Resolution|resolution]] 3.28Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4csk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CSK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4csk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CSK FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4csk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4csk OCA], [http://pdbe.org/4csk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4csk RCSB], [http://www.ebi.ac.uk/pdbsum/4csk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4csk ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.28Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4csk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4csk OCA], [https://pdbe.org/4csk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4csk RCSB], [https://www.ebi.ac.uk/pdbsum/4csk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4csk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/AQP1_HUMAN AQP1_HUMAN]] Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.<ref>PMID:1373524</ref> | + | [https://www.uniprot.org/uniprot/AQP1_HUMAN AQP1_HUMAN] Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.<ref>PMID:1373524</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cornvik, T]] | + | [[Category: Cornvik T]] |
| - | [[Category: Darwis, D]] | + | [[Category: Darwis D]] |
| - | [[Category: Lescar, J]] | + | [[Category: Lescar J]] |
| - | [[Category: Ruiz-Carrillo, D]] | + | [[Category: Ruiz-Carrillo D]] |
| - | [[Category: Soon, C H]] | + | [[Category: Soon CH]] |
| - | [[Category: To-Yiu-Ying, J]] | + | [[Category: To-Yiu-Ying J]] |
| - | [[Category: Torres, J]] | + | [[Category: Torres J]] |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
AQP1_HUMAN Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.[1]
Publication Abstract from PubMed
Aquaporin water channels (AQPs) are found in almost every organism from humans to bacteria. In humans, 13 classes of AQPs control water and glycerol homeostasis. Knockout studies have suggested that modulating the activity of AQPs could be beneficial for the treatment of several pathologies. In particular, aquaporin 1 is a key factor in cell migration and angiogenesis, and constitutes a possible target for anticancer compounds and also for the treatment of glaucoma. Here, a preliminary crystallographic analysis at 3.28 A resolution of crystals of human aquaporin 1 (hAQP1) obtained from protein expressed in Sf9 insect cells is reported. The crystals belonged to the tetragonal space group I422, with unit-cell parameters a = b = 89.28, c = 174.9 A, and contained one monomer per asymmetric unit. The hAQP1 biological tetramer is generated via the crystallographic fourfold axis. This work extends previous electron crystallographic studies that used material extracted from human red blood cells, in which the resolution was limited to approximately 3.8 A. It will inform efforts to improve lattice contacts and the diffraction limit for the future structure-based discovery of specific hAQP1 inhibitors.
Crystallization and preliminary crystallographic analysis of human aquaporin 1 at a resolution of 3.28 A.,Ruiz Carrillo D, To Yiu Ying J, Darwis D, Soon CH, Cornvik T, Torres J, Lescar J Acta Crystallogr F Struct Biol Commun. 2014 Dec 1;70(Pt 12):1657-63. doi:, 10.1107/S2053230X14024558. Epub 2014 Nov 14. PMID:25484221[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Preston GM, Carroll TP, Guggino WB, Agre P. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science. 1992 Apr 17;256(5055):385-7. PMID:1373524
- ↑ Ruiz Carrillo D, To Yiu Ying J, Darwis D, Soon CH, Cornvik T, Torres J, Lescar J. Crystallization and preliminary crystallographic analysis of human aquaporin 1 at a resolution of 3.28 A. Acta Crystallogr F Struct Biol Commun. 2014 Dec 1;70(Pt 12):1657-63. doi:, 10.1107/S2053230X14024558. Epub 2014 Nov 14. PMID:25484221 doi:http://dx.doi.org/10.1107/S2053230X14024558
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