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4d0a

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Current revision

3D EM map of the sodium proton antiporter MjNhaP1 from Methanocaldococcus jannaschii

Structural highlights

4d0a is a 1 chain structure with sequence from Methanocaldococcus jannaschii DSM 2661. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron crystallography, Resolution 6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NAH1_METJA This is a Na(+)/H(+) antiporter. Can also transport lithium.^[1]

Publication Abstract from PubMed

Sodium/proton antiporters are essential for sodium and pH homeostasis and play a major role in human health and disease. We determined the structures of the archaeal sodium/proton antiporter MjNhaP1 in two complementary states. The inward-open state was obtained by x-ray crystallography in the presence of sodium at pH8, where the transporter is highly active. The outward-open state was obtained by electron crystallography without sodium at pH4, where MjNhaP1 is inactive. Comparison of both structures reveals a 7{degree sign} tilt of the 6 helix bundle. 22Na+ uptake measurements indicate non-cooperative transport with an activity maximum at pH7.5. We conclude that binding of a Na+ ion from the outside induces helix movements that close the extracellular cavity, open the cytoplasmic funnel, and result in a ~5 A vertical relocation of the ion binding site to release the substrate ion into the cytoplasm.

Structure and transport mechanism of the sodium/protonantiporter MjNhaP1.,Paulino C, Wohlert D, Kapotova E, Yildiz O, Kuhlbrandt W Elife. 2014 Nov 26;3. doi: 10.7554/eLife.03583. PMID:25426803^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hellmer J, Patzold R, Zeilinger C. Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii. FEBS Lett. 2002 Sep 11;527(1-3):245-9. PMID:12220668
↑ Paulino C, Wohlert D, Kapotova E, Yildiz O, Kuhlbrandt W. Structure and transport mechanism of the sodium/protonantiporter MjNhaP1. Elife. 2014 Nov 26;3. doi: 10.7554/eLife.03583. PMID:25426803 doi:http://dx.doi.org/10.7554/eLife.03583

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4d0a"

@@ Line 3: / Line 3: @@
 <StructureSection load='4d0a' size='340' side='right'caption='[[4d0a]], [[Resolution|resolution]] 6.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4d0a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Metja Metja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D0A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4d0a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D0A FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d0a OCA], [http://pdbe.org/4d0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d0a RCSB], [http://www.ebi.ac.uk/pdbsum/4d0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d0a ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 6&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d0a OCA], [https://pdbe.org/4d0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d0a RCSB], [https://www.ebi.ac.uk/pdbsum/4d0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d0a ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/NAH1_METJA NAH1_METJA]] This is a Na(+)/H(+) antiporter. Can also transport lithium.<ref>PMID:12220668</ref>
+[https://www.uniprot.org/uniprot/NAH1_METJA NAH1_METJA] This is a Na(+)/H(+) antiporter. Can also transport lithium.<ref>PMID:12220668</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Metja]]
+[[Category: Methanocaldococcus jannaschii DSM 2661]]
-[[Category: Kuhlbrandt, W]]
+[[Category: Kuhlbrandt W]]
-[[Category: Paulino, C]]
+[[Category: Paulino C]]
-[[Category: Woehlert, D]]
+[[Category: Woehlert D]]
-[[Category: Yildiz, O]]
+[[Category: Yildiz O]]
-[[Category: Antiporter]]
-[[Category: Cpa]]
-[[Category: Exchanger]]
-[[Category: Membrane protein]]
-[[Category: Transport protein]]
-[[Category: Transporter]]

4d0a

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Current revision

3D EM map of the sodium proton antiporter MjNhaP1 from Methanocaldococcus jannaschii

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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