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| | <StructureSection load='4cxf' size='340' side='right'caption='[[4cxf]], [[Resolution|resolution]] 1.75Å' scene=''> | | <StructureSection load='4cxf' size='340' side='right'caption='[[4cxf]], [[Resolution|resolution]] 1.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4cxf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupmc Cupmc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CXF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4cxf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans_CH34 Cupriavidus metallidurans CH34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CXF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxf OCA], [http://pdbe.org/4cxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cxf RCSB], [http://www.ebi.ac.uk/pdbsum/4cxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cxf ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxf OCA], [https://pdbe.org/4cxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cxf RCSB], [https://www.ebi.ac.uk/pdbsum/4cxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cxf ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CNRH_RALME CNRH_RALME]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates the genes for a membrane-located efflux system that confers resistance to nickel and cobalt. CnrH alone is able to activate CNR expression, while both CnrY and CrnX are needed for nickel induction of cnrH (PubMed:10671463). Binds DNA in an RNA polymerase-dependent fashion (PubMed:10671464). CnrH may be controlled by a CnrYX transmembrane anti-sigma factor complex which binds CnrH in the absence of Ni(2+). If Ni(2+) appears in the periplasm, it may be bound by CnrR (CnrX); the signal then would be transmitted by CnrY into the cytoplasm and CnrH would be released. [[http://www.uniprot.org/uniprot/CNRY_RALME CNRY_RALME]] Nickel and cobalt resistance proteins CnrA, CnrB, CnrC CnrH and CnrR may be involved in the regulation of CNR. CnrH alone is able to activate cnr expression, and both CnrY and CrnX are needed for nickel induction of CnrH (PubMed:10671463). In the absence of wild-type CnrY (due either to a frameshift, PubMed:10671463 or absence of the transcript, PubMed:10671464), nickel and cobalt resistance is constitutive, indicating that CrnY may act as a repressor (PubMed:10671463) or an anti-sigma factor (PubMed:10671464). | + | [https://www.uniprot.org/uniprot/CNRH_CUPMC CNRH_CUPMC] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates the genes for a membrane-located efflux system that confers resistance to nickel and cobalt. CnrH alone is able to activate CNR expression, while both CnrY and CrnX are needed for nickel induction of cnrH (PubMed:10671463). Binds DNA in an RNA polymerase-dependent fashion (PubMed:10671464). CnrH may be controlled by a CnrYX transmembrane anti-sigma factor complex which binds CnrH in the absence of Ni(2+). If Ni(2+) appears in the periplasm, it may be bound by CnrR (CnrX); the signal then would be transmitted by CnrY into the cytoplasm and CnrH would be released.<ref>PMID:10671463</ref> <ref>PMID:10671464</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Sigma factor|Sigma factor]] | + | *[[Sigma factor 3D structures|Sigma factor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cupmc]] | + | [[Category: Cupriavidus metallidurans CH34]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Coves, J]] | + | [[Category: Coves J]] |
| - | [[Category: Girard, E]] | + | [[Category: Girard E]] |
| - | [[Category: Kahn, R]] | + | [[Category: Kahn R]] |
| - | [[Category: Maillard, A P]] | + | [[Category: Maillard AP]] |
| - | [[Category: Petit-Hartlein, I]] | + | [[Category: Petit-Hartlein I]] |
| - | [[Category: Ziani, W]] | + | [[Category: Ziani W]] |
| - | [[Category: Antisigma]]
| + | |
| - | [[Category: Ecf-type sigma]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
CNRH_CUPMC Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates the genes for a membrane-located efflux system that confers resistance to nickel and cobalt. CnrH alone is able to activate CNR expression, while both CnrY and CrnX are needed for nickel induction of cnrH (PubMed:10671463). Binds DNA in an RNA polymerase-dependent fashion (PubMed:10671464). CnrH may be controlled by a CnrYX transmembrane anti-sigma factor complex which binds CnrH in the absence of Ni(2+). If Ni(2+) appears in the periplasm, it may be bound by CnrR (CnrX); the signal then would be transmitted by CnrY into the cytoplasm and CnrH would be released.[1] [2]
Publication Abstract from PubMed
Gene expression in bacteria is regulated at the level of transcription initiation, a process driven by sigma factors. The regulation of sigma factor activity proceeds from the regulation of their cytoplasmic availability, which relies on specific inhibitory proteins called anti-sigma factors. With anti-sigma factors regulating their availability according to diverse cues, extracytoplasmic function sigma factors (sigmaECF) form a major signal transduction system in bacteria. Here, structure:function relationships have been characterized in an emerging class of minimal-size transmembrane anti-sigma factors, using CnrY from Cupriavidus metallidurans CH34 as a model. This study reports the 1.75-A-resolution structure of CnrY cytosolic domain in complex with CnrH, its cognate sigmaECF, and identifies a small hydrophobic knob in CnrY as the major determinant of this interaction in vivo. Unsuspected structural similarity with the molecular switch regulating the general stress response in alpha-proteobacteria unravels a new class of anti-sigma factors targeting sigmaECF. Members of this class carry out their function via a 30-residue stretch that displays helical propensity but no canonical structure on its own.
The Crystal Structure of the Anti-sigma Factor CnrY in Complex with the sigma Factor CnrH Shows a New Structural Class of Anti-sigma Factors Targeting Extracytoplasmic Function sigma Factors.,Maillard AP, Girard E, Ziani W, Petit-Hartlein I, Kahn R, Coves J J Mol Biol. 2014 Apr 12. pii: S0022-2836(14)00177-6. doi:, 10.1016/j.jmb.2014.04.003. PMID:24727125[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Grass G, Grosse C, Nies DH. Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34. J Bacteriol. 2000 Mar;182(5):1390-8. PMID:10671463
- ↑ Tibazarwa C, Wuertz S, Mergeay M, Wyns L, van Der Lelie D. Regulation of the cnr cobalt and nickel resistance determinant of Ralstonia eutropha (Alcaligenes eutrophus) CH34. J Bacteriol. 2000 Mar;182(5):1399-409. PMID:10671464
- ↑ Maillard AP, Girard E, Ziani W, Petit-Hartlein I, Kahn R, Coves J. The Crystal Structure of the Anti-sigma Factor CnrY in Complex with the sigma Factor CnrH Shows a New Structural Class of Anti-sigma Factors Targeting Extracytoplasmic Function sigma Factors. J Mol Biol. 2014 Apr 12. pii: S0022-2836(14)00177-6. doi:, 10.1016/j.jmb.2014.04.003. PMID:24727125 doi:http://dx.doi.org/10.1016/j.jmb.2014.04.003
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