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| | ==Dengue 1 cryo-EM reconstruction== | | ==Dengue 1 cryo-EM reconstruction== |
| - | <StructureSection load='4cct' size='340' side='right'caption='[[4cct]], [[Resolution|resolution]] 4.50Å' scene=''> | + | <SX load='4cct' size='340' side='right' viewer='molstar' caption='[[4cct]], [[Resolution|resolution]] 4.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4cct]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_1 Dengue virus 1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4azx 4azx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CCT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CCT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4cct]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_1 Dengue virus 1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4azx 4azx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CCT FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4b03|4b03]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cct OCA], [http://pdbe.org/4cct PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cct RCSB], [http://www.ebi.ac.uk/pdbsum/4cct PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cct ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cct OCA], [https://pdbe.org/4cct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cct RCSB], [https://www.ebi.ac.uk/pdbsum/4cct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cct ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/G3F5K5_9FLAV G3F5K5_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774] | + | [https://www.uniprot.org/uniprot/G3F5K5_9FLAV G3F5K5_9FLAV] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| | [[Category: Dengue virus 1]] | | [[Category: Dengue virus 1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kostyuchenko, V A]] | + | [[Category: Kostyuchenko VA]] |
| - | [[Category: Lok, S M]] | + | [[Category: Lok SM]] |
| - | [[Category: Ng, T S]] | + | [[Category: Ng TS]] |
| - | [[Category: Tan, J L]] | + | [[Category: Tan JL]] |
| - | [[Category: Zhang, Q]] | + | [[Category: Zhang Q]] |
| - | [[Category: Flavivirus]]
| + | |
| - | [[Category: Virus]]
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| Structural highlights
Function
G3F5K5_9FLAV Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774]
Publication Abstract from PubMed
Dengue virus is a major human pathogen that has four serotypes (DENV1 to -4). Here we report the cryoelectron microscopy (cryo-EM) structures of immature and mature DENV1 at 6- and 4.5-A resolution, respectively. The subnanometer-resolution maps allow accurate placement of all of the surface proteins. Although the immature and mature viruses showed vastly different surface protein organizations, the envelope protein transmembrane (E-TM) regions remain in similar positions. The pivotal role of the E-TM regions leads to the identification of the start and end positions of all surface proteins during maturation.
Immature and mature dengue serotype 1 virus structures provide insight into the maturation process.,Kostyuchenko VA, Zhang Q, Tan JL, Ng TS, Lok SM J Virol. 2013 Jul;87(13):7700-7. doi: 10.1128/JVI.00197-13. Epub 2013 May 1. PMID:23637416[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kostyuchenko VA, Zhang Q, Tan JL, Ng TS, Lok SM. Immature and mature dengue serotype 1 virus structures provide insight into the maturation process. J Virol. 2013 Jul;87(13):7700-7. doi: 10.1128/JVI.00197-13. Epub 2013 May 1. PMID:23637416 doi:10.1128/JVI.00197-13
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