2qir

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[[Image:2qir.jpg|left|200px]]
 
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==Crystal structure of aminoglycoside acetyltransferase AAC(6')-Ib in complex whith coenzyme A and kanamycin==
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The line below this paragraph, containing "STRUCTURE_2qir", creates the "Structure Box" on the page.
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<StructureSection load='2qir' size='340' side='right'caption='[[2qir]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2qir]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QIR FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9CS:(1R,2S,3S,4R,6S)-4,6-DIAMINO-3-[(3-AMINO-3-DEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-2-HYDROXYCYCLOHEXYL+2,6-DIAMINO-2,6-DIDEOXY-ALPHA-D-GLUCOPYRANOSIDE'>9CS</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2prb|2prb]], [[2pr8|2pr8]]</div></td></tr>
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{{STRUCTURE_2qir| PDB=2qir | SCENE= }}
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Aminoglycoside_N(6')-acetyltransferase Aminoglycoside N(6')-acetyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.82 2.3.1.82] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qir OCA], [https://pdbe.org/2qir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qir RCSB], [https://www.ebi.ac.uk/pdbsum/2qir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qir ProSAT]</span></td></tr>
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'''Crystal structure of aminoglycoside acetyltransferase AAC(6')-Ib in complex whith coenzyme A and kanamycin'''
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qi/2qir_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qir ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The emergence of multi-resistant pathogenic bacteria is a worldwide health issue. Recently, clinical variants of a single antibiotic-modifying acetyltransferase, AAC(6')-Ib-a variant of aminoglycoside 6'-N-acetyltransferase-have been identified that confer extended resistance to most aminoglycosides and, more surprisingly, to structurally unrelated fluoroquinolones. The corresponding gene is carried by mobile genetic elements and is present in most multi-resistant pathogenic strains, hence making it a serious threat to current therapies. Here, we report the crystal structures of both narrow- and broad-spectrum resistance variants of this enzyme, which reveal the structural basis for the emergence of extended resistance. The active site shows an important plasticity and has adapted to new substrates by a large-scale gaping process. We have also obtained co-crystals with both substrates, and with a simple transition state analogue, which provides new clues for the design of inhibitors of this resistance mechanism.
The emergence of multi-resistant pathogenic bacteria is a worldwide health issue. Recently, clinical variants of a single antibiotic-modifying acetyltransferase, AAC(6')-Ib-a variant of aminoglycoside 6'-N-acetyltransferase-have been identified that confer extended resistance to most aminoglycosides and, more surprisingly, to structurally unrelated fluoroquinolones. The corresponding gene is carried by mobile genetic elements and is present in most multi-resistant pathogenic strains, hence making it a serious threat to current therapies. Here, we report the crystal structures of both narrow- and broad-spectrum resistance variants of this enzyme, which reveal the structural basis for the emergence of extended resistance. The active site shows an important plasticity and has adapted to new substrates by a large-scale gaping process. We have also obtained co-crystals with both substrates, and with a simple transition state analogue, which provides new clues for the design of inhibitors of this resistance mechanism.
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==About this Structure==
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Enzyme structural plasticity and the emergence of broad-spectrum antibiotic resistance.,Maurice F, Broutin I, Podglajen I, Benas P, Collatz E, Dardel F EMBO Rep. 2008 Apr;9(4):344-9. Epub 2008 Feb 22. PMID:18292754<ref>PMID:18292754</ref>
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2QIR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QIR OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Enzyme structural plasticity and the emergence of broad-spectrum antibiotic resistance., Maurice F, Broutin I, Podglajen I, Benas P, Collatz E, Dardel F, EMBO Rep. 2008 Apr;9(4):344-349. Epub 2008 Feb 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18292754 18292754]
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</div>
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[[Category: Salmonella typhimurium]]
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<div class="pdbe-citations 2qir" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Benas, P.]]
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<references/>
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[[Category: Broutin, I.]]
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__TOC__
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[[Category: Collatz, E.]]
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</StructureSection>
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[[Category: Dardel, F.]]
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[[Category: Large Structures]]
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[[Category: Maurice, F.]]
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[[Category: Benas, P]]
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[[Category: Podglajen, I.]]
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[[Category: Broutin, I]]
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[[Category: Collatz, E]]
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[[Category: Dardel, F]]
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[[Category: Maurice, F]]
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[[Category: Podglajen, I]]
[[Category: Acetyltransferase]]
[[Category: Acetyltransferase]]
[[Category: Aminoglycoside resistance]]
[[Category: Aminoglycoside resistance]]
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[[Category: Transferase]]

Current revision

Crystal structure of aminoglycoside acetyltransferase AAC(6')-Ib in complex whith coenzyme A and kanamycin

PDB ID 2qir

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