6jic
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Identification and Characterization of a carboxypeptidase inhibitor from Lycium barbarum== | |
| + | <StructureSection load='6jic' size='340' side='right'caption='[[6jic]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6jic]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lycium_barbarum Lycium barbarum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JIC FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jic OCA], [https://pdbe.org/6jic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jic RCSB], [https://www.ebi.ac.uk/pdbsum/6jic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jic ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hyperstable cysteine-rich peptides (CRPs) represent an underexplored superfamily of bioactives in functional foods. An example is wolfberry of the Lycium barbarum family. Previously, we discovered a CRP, designated alpha-lybatide, from L. barbarum bark. Herein, we report the discovery of beta-lybatide, a novel carboxypeptidase inhibitor belonging to a different CRP family from the wolfberry plant. Proteomic and transcriptomic analyses showed that beta-lybatide contains 36 amino acids with six cysteine residues. NMR spectroscopy revealed that beta-lybatide displays a knottin-like structure that renders it highly resistant to thermal, chemical and enzymatic degradation, conditions important for keeping its structural integrity in gastrointestinal tract. Biochemical assays showed that beta-lybatide is a potent carboxypeptidase inhibitor which could contribute to the wolfberry biological activities. Bioinformatics analysis revealed an additional 49 beta-lybatide-like plant carboxypeptidase inhibitors. Together, our results show that beta-lybatide is the first and the smallest plant-derived hyperstable carboxypeptidase inhibitor discovered from a functional food. | ||
| - | + | Identification and characterization of a wolfberry carboxypeptidase inhibitor from Lycium barbarum.,Huang J, Wong KH, Tan WL, Tay SV, Wang S, Tam JP Food Chem. 2021 Feb 19;351:129338. doi: 10.1016/j.foodchem.2021.129338. PMID:33647700<ref>PMID:33647700</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6jic" style="background-color:#fffaf0;"></div> |
| - | [[Category: Tan | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Wong | + | </StructureSection> |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Lycium barbarum]] | ||
| + | [[Category: Huang JY]] | ||
| + | [[Category: Tan WL]] | ||
| + | [[Category: Tay SV]] | ||
| + | [[Category: Wang SJ]] | ||
| + | [[Category: Wong KH]] | ||
Current revision
Identification and Characterization of a carboxypeptidase inhibitor from Lycium barbarum
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Categories: Large Structures | Lycium barbarum | Huang JY | Tan WL | Tay SV | Wang SJ | Wong KH
