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| | <StructureSection load='4h3m' size='340' side='right'caption='[[4h3m]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='4h3m' size='340' side='right'caption='[[4h3m]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4h3m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp._lw-2004 Discosoma sp. lw-2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H3M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4h3m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Discosoma_sp._LW-2004 Discosoma sp. LW-2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H3M FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4h3l|4h3l]], [[4h3n|4h3n]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3m OCA], [http://pdbe.org/4h3m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h3m RCSB], [http://www.ebi.ac.uk/pdbsum/4h3m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h3m ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3m OCA], [https://pdbe.org/4h3m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h3m RCSB], [https://www.ebi.ac.uk/pdbsum/4h3m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h3m ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5S3G7_9CNID Q5S3G7_9CNID] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Discosoma sp. lw-2004]] | + | [[Category: Discosoma sp. LW-2004]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chica, R A]] | + | [[Category: Chica RA]] |
| - | [[Category: Moore, M M]] | + | [[Category: Moore MM]] |
| - | [[Category: Fluorescent protein]]
| + | |
| Structural highlights
Function
Q5S3G7_9CNID
Publication Abstract from PubMed
Red fluorescent proteins (RFPs) derived from organisms in the class Anthozoa have found widespread application as imaging tools in biological research. For most imaging experiments, RFPs that mature quickly to the red chromophore and produce little or no green chromophore are most useful. In this study, we used rational design to convert a yellow fluorescent mPlum mutant to a red-emitting RFP without reverting any of the mutations causing the maturation deficiency and without altering the red chromophore's covalent structure. We also created an optimized mPlum mutant (mPlum-E16P) that matures almost exclusively to the red chromophore. Analysis of the structure/function relationships in these proteins revealed two structural characteristics that are important for efficient red chromophore maturation in DsRed-derived RFPs. The first is the presence of a lysine residue at position 70 that is able to interact directly with the chromophore. The second is an absence of non-bonding interactions limiting the conformational flexibility at the peptide backbone that is oxidized during red chromophore formation. Satisfying or improving these structural features in other maturation-deficient RFPs may result in RFPs with faster and more complete maturation to the red chromophore.
Recovery of Red Fluorescent Protein Chromophore Maturation Deficiency through Rational Design.,Moore MM, Oteng-Pabi SK, Pandelieva AT, Mayo SL, Chica RA PLoS One. 2012;7(12):e52463. doi: 10.1371/journal.pone.0052463. Epub 2012 Dec 20. PMID:23285050[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Moore MM, Oteng-Pabi SK, Pandelieva AT, Mayo SL, Chica RA. Recovery of Red Fluorescent Protein Chromophore Maturation Deficiency through Rational Design. PLoS One. 2012;7(12):e52463. doi: 10.1371/journal.pone.0052463. Epub 2012 Dec 20. PMID:23285050 doi:http://dx.doi.org/10.1371/journal.pone.0052463
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