4f6t

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<StructureSection load='4f6t' size='340' side='right'caption='[[4f6t]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
<StructureSection load='4f6t' size='340' side='right'caption='[[4f6t]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4f6t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F6T FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4f6t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F6T FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6M0:MO(6)-O(26)+CLUSTER'>6M0</scene>, <scene name='pdbligand=8M0:BIS(MU4-OXO)-TETRAKIS(MU3-OXO)-HEXAKIS(MU2-OXO)-HEXADECAOXO-OCTAMOLYBDENUM+(VI)'>8M0</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ogx|2ogx]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6M0:MO(6)-O(26)+CLUSTER'>6M0</scene>, <scene name='pdbligand=8M0:BIS(MU4-OXO)-TETRAKIS(MU3-OXO)-HEXAKIS(MU2-OXO)-HEXADECAOXO-OCTAMOLYBDENUM+(VI)'>8M0</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f6t OCA], [http://pdbe.org/4f6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f6t RCSB], [http://www.ebi.ac.uk/pdbsum/4f6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f6t ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f6t OCA], [https://pdbe.org/4f6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f6t RCSB], [https://www.ebi.ac.uk/pdbsum/4f6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f6t ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/MOSB_AZOVD MOSB_AZOVD]] Intracellular storage of molybdenum. Binds polyoxomolybdates. Can bind at least 90 molybdenum atoms per protein molecule. [[http://www.uniprot.org/uniprot/MOSA_AZOVD MOSA_AZOVD]] Intracellular storage of molybdenum. Binds polyoxomolybdates. Can bind at least 90 molybdenum atoms per protein molecule.
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[https://www.uniprot.org/uniprot/MOSB_AZOVD MOSB_AZOVD] Intracellular storage of molybdenum. Binds polyoxomolybdates. Can bind at least 90 molybdenum atoms per protein molecule.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Some N(2)-fixing bacteria prolong the functionality of nitrogenase in molybdenum starvation by a special Mo storage protein (MoSto) that can store more than 100 Mo atoms. The presented 1.6 A X-ray structure of MoSto from Azotobacter vinelandii reveals various discrete polyoxomolybdate clusters, three covalently and three noncovalently bound Mo(8), three Mo(5-7), and one Mo(3) clusters, and several low occupied, so far undefinable clusters, which are embedded in specific pockets inside a locked cage-shaped (alphabeta)(3) protein complex. The structurally identical Mo(8) clusters (three layers of two, four, and two MoO(n) octahedra) are distinguishable from the [Mo(8)O(26)](4-) cluster formed in acidic solutions by two displaced MoO(n) octahedra implicating three kinetically labile terminal ligands. Stabilization in the covalent Mo(8) cluster is achieved by Mo bonding to Hisalpha156-N(epsilon2) and Glualpha129-O(epsilon1). The absence of covalent protein interactions in the noncovalent Mo(8) cluster is compensated by a more extended hydrogen-bond network involving three pronounced histidines. One displaced MoO(n) octahedron might serve as nucleation site for an inhomogeneous Mo(5-7) cluster largely surrounded by bulk solvent. In the Mo(3) cluster located on the 3-fold axis, the three accurately positioned His140-N(epsilon2) atoms of the alpha subunits coordinate to the Mo atoms. The formed polyoxomolybdate clusters of MoSto, not detectable in bulk solvent, are the result of an interplay between self- and protein-driven assembly processes that unite inorganic supramolecular and protein chemistry in a host-guest system. Template, nucleation/protection, and catalyst functions of the polypeptide as well as perspectives for designing new clusters are discussed.
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Nature's Polyoxometalate Chemistry: X-ray Structure of the Mo Storage Protein Loaded with Discrete Polynuclear Mo-O Clusters.,Kowalewski B, Poppe J, Demmer U, Warkentin E, Dierks T, Ermler U, Schneider K J Am Chem Soc. 2012 Jun 13;134(23):9768-74. Epub 2012 May 31. PMID:22612644<ref>PMID:22612644</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4f6t" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Azotobacter vinelandii]]
[[Category: Azotobacter vinelandii]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Demmer, U]]
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[[Category: Demmer U]]
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[[Category: Ermler, U]]
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[[Category: Ermler U]]
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[[Category: Kowalewski, B]]
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[[Category: Kowalewski B]]
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[[Category: Poppe, J]]
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[[Category: Poppe J]]
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[[Category: Schneider, K]]
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[[Category: Schneider K]]
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[[Category: Warkentin, E]]
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[[Category: Warkentin E]]
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[[Category: Atp hydrolysis]]
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[[Category: Atp hydrolyzation]]
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[[Category: Hexamer]]
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[[Category: Metal binding protein]]
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[[Category: Molybdate]]
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[[Category: Molybdenum storage]]
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[[Category: Polyoxometalate]]
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[[Category: Pom]]
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[[Category: Rossmanfold like]]
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Current revision

The crystal structure of the molybdenum storage protein (MoSto) from Azotobacter vinelandii loaded with various polyoxometalates

PDB ID 4f6t

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