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| | <StructureSection load='4d81' size='340' side='right'caption='[[4d81]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='4d81' size='340' side='right'caption='[[4d81]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4d81]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_51363 Atcc 51363]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D81 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D81 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4d81]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Metallosphaera_sedula Metallosphaera sedula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D81 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4d82|4d82]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d81 OCA], [https://pdbe.org/4d81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d81 RCSB], [https://www.ebi.ac.uk/pdbsum/4d81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d81 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d81 OCA], [http://pdbe.org/4d81 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d81 RCSB], [http://www.ebi.ac.uk/pdbsum/4d81 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d81 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A4YHC5_METS5 A4YHC5_METS5] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 51363]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Vesicle-fusing ATPase]] | + | [[Category: Metallosphaera sedula]] |
| - | [[Category: Boeri-Erba, E]] | + | [[Category: Boeri-Erba E]] |
| - | [[Category: Caillat, C]] | + | [[Category: Caillat C]] |
| - | [[Category: Effantin, G]] | + | [[Category: Effantin G]] |
| - | [[Category: Gottlinger, H G]] | + | [[Category: Gottlinger HG]] |
| - | [[Category: Macheboeuf, P]] | + | [[Category: Macheboeuf P]] |
| - | [[Category: McCarthy, A A]] | + | [[Category: McCarthy AA]] |
| - | [[Category: Renesto, P]] | + | [[Category: Renesto P]] |
| - | [[Category: Weissenhorn, W]] | + | [[Category: Weissenhorn W]] |
| - | [[Category: Wu, Y]] | + | [[Category: Wu Y]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Vps4]]
| + | |
| Structural highlights
Function
A4YHC5_METS5
Publication Abstract from PubMed
The vacuolar protein sorting 4 AAA-ATPase (Vps4) recycles endosomal sorting complexes required for transport (ESCRT-III) polymers from cellular membranes. Here we present a 3.6-A X-ray structure of ring-shaped Vps4 from Metallosphera sedula (MsVps4), seen as an asymmetric pseudohexamer. Conserved key interface residues are shown to be important for MsVps4 assembly, ATPase activity in vitro, ESCRT-III disassembly in vitro and HIV-1 budding. ADP binding leads to conformational changes within the protomer, which might propagate within the ring structure. All ATP-binding sites are accessible and the pseudohexamer binds six ATP with micromolar affinity in vitro. In contrast, ADP occupies one high-affinity and five low-affinity binding sites in vitro, consistent with conformational asymmetry induced on ATP hydrolysis. The structure represents a snapshot of an assembled Vps4 conformation and provides insight into the molecular motions the ring structure undergoes in a concerted action to couple ATP hydrolysis to ESCRT-III substrate disassembly.
Asymmetric ring structure of Vps4 required for ESCRT-III disassembly.,Caillat C, Macheboeuf P, Wu Y, McCarthy AA, Boeri-Erba E, Effantin G, Gottlinger HG, Weissenhorn W, Renesto P Nat Commun. 2015 Dec 3;6:8781. doi: 10.1038/ncomms9781. PMID:26632262[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Caillat C, Macheboeuf P, Wu Y, McCarthy AA, Boeri-Erba E, Effantin G, Gottlinger HG, Weissenhorn W, Renesto P. Asymmetric ring structure of Vps4 required for ESCRT-III disassembly. Nat Commun. 2015 Dec 3;6:8781. doi: 10.1038/ncomms9781. PMID:26632262 doi:http://dx.doi.org/10.1038/ncomms9781
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