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4e51

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<StructureSection load='4e51' size='340' side='right'caption='[[4e51]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
<StructureSection load='4e51' size='340' side='right'caption='[[4e51]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burta Burta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E51 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4e51]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_thailandensis_E264 Burkholderia thailandensis E264]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E51 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E51 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BTH_I2235, hisS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271848 BURTA])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine--tRNA_ligase Histidine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.21 6.1.1.21] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [https://pdbe.org/4e51 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [https://www.ebi.ac.uk/pdbsum/4e51 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e51 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e51 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e51 OCA], [http://pdbe.org/4e51 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e51 RCSB], [http://www.ebi.ac.uk/pdbsum/4e51 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e51 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/SYH_BURTA SYH_BURTA]
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Aminoacyl-tRNA synthetases (aaRSs) charge tRNAs with their cognate amino acid, an essential precursor step to loading of charged tRNAs onto the ribosome and addition of the amino acid to the growing polypeptide chain during protein synthesis. Because of this important biological function, aminoacyl-tRNA synthetases have been the focus of anti-infective drug development efforts and two aaRS inhibitors have been approved as drugs. Several researchers in the scientific community requested aminoacyl-tRNA synthetases to be targeted in the Seattle Structural Genomics Center for Infectious Disease (SSGCID) structure determination pipeline. Here we investigate thirty-one aminoacyl-tRNA synthetases from infectious disease organisms by co-crystallization in the presence of their cognate amino acid, ATP, and/or inhibitors. Crystal structures were determined for a CysRS from Borrelia burgdorferi bound to AMP, GluRS from Borrelia burgdorferi and Burkholderia thailandensis bound to glutamic acid, a TrpRS from the eukaryotic pathogen Encephalitozoon cuniculi bound to tryptophan, a HisRS from Burkholderia thailandensis bound to histidine, and a LysRS from Burkholderia thailandensis bound to lysine. Thus, the presence of ligands may promote aaRS crystallization and structure determination. Comparison with homologous structures shows conformational flexibility that appears to be a recurring theme with this enzyme class.
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Ligand co-crystallization of aminoacyl-tRNA synthetases from infectious disease organisms.,Moen SO, Edwards TE, Dranow DM, Clifton MC, Sankaran B, Van Voorhis WC, Sharma A, Manoil C, Staker BL, Myler PJ, Lorimer DD Sci Rep. 2017 Mar 16;7(1):223. doi: 10.1038/s41598-017-00367-6. PMID:28303005<ref>PMID:28303005</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4e51" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Burta]]
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[[Category: Burkholderia thailandensis E264]]
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[[Category: Histidine--tRNA ligase]]
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Structural genomic]]
 
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[[Category: Aar]]
 
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[[Category: Aminoacylation]]
 
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[[Category: Atp-dependent]]
 
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[[Category: Charged trna]]
 
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[[Category: Histidyl-adenylate]]
 
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[[Category: Ligase]]
 
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[[Category: Ssgcid]]
 
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[[Category: Trna activation]]
 
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[[Category: Trna synthetase]]
 

Current revision

Crystal structure of a histidyl-tRNA synthetase HisRS from Burkholderia thailandensis bound to histidine

PDB ID 4e51

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