4heq

<table><tr><td colspan='2'>[[4heq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_gigas Desulfovibrio gigas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HEQ FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4heq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4heq OCA], [http://pdbe.org/4heq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4heq RCSB], [http://www.ebi.ac.uk/pdbsum/4heq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4heq ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Flavodoxins, which exist widely in microorganisms, have been found in various pathways with multiple physiological functions. The flavodoxin (Fld) containing the cofactor flavin mononucleotide (FMN) from sulfur-reducing bacteria Desulfovibrio gigas (D. gigas) is a short-chain enzyme that comprises 146 residues with a molecular mass of 15 kDa and plays important roles in the electron-transfer chain. To investigate its structure, we purified this Fld directly from anaerobically grown D. gigas cells. The crystal structure of Fld, determined at resolution 1.3 A, is a dimer with two FMN packing in an orientation head to head at a distance of 17 A, which generates a long and connected negatively charged region. Two loops, Thr59-Asp63 and Asp95-Tyr100, are located in the negatively charged region and between two FMN, and are structurally dynamic. An analysis of each monomer shows that the structure of Fld is in a semiquinone state; the positions of FMN and the surrounding residues in the active site deviate. The crystal structure of Fld from D. gigas agrees with a dimeric form in the solution state. The dimerization area, dynamic characteristics and structure variations between monomers enable us to identify a possible binding area for its functional partners.

 

 

*[[Flavodoxin|Flavodoxin]]

[[Category: Desulfovibrio gigas]]

[[Category: Chen, C J]]

[[Category: Hsieh, Y C]]

[[Category: Cytoplasma]]

[[Category: Electron transport]]