6j5u

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==Ligand-triggered allosteric ADP release primes a plant NLR complex==
==Ligand-triggered allosteric ADP release primes a plant NLR complex==
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<StructureSection load='6j5u' size='340' side='right'caption='[[6j5u]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
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<SX load='6j5u' size='340' side='right' viewer='molstar' caption='[[6j5u]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6j5u]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J5U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J5U FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6j5u]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J5U FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BQL:[(2~{S})-2-azanyl-3-oxidanylidene-propyl]+[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl+hydrogen+phosphate'>BQL</scene>, <scene name='pdbligand=BQO:[(2~{R},3~{S})-3-azanyl-4-oxidanylidene-butan-2-yl]+[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl+hydrogen+phosphate'>BQO</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j5u OCA], [https://pdbe.org/6j5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j5u RCSB], [https://www.ebi.ac.uk/pdbsum/6j5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j5u ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j5u OCA], [http://pdbe.org/6j5u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j5u RCSB], [http://www.ebi.ac.uk/pdbsum/6j5u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j5u ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/R13L4_ARATH R13L4_ARATH]] CC-NB-LRR receptor-like protein required for recognition of the Pseudomonas syringae type III effector HopZ1a. Confers allele-specific recognition and virulence attenuation of HopZ1a. Immunity mediated by ZAR1 is independent of several genes required by other resistance protein signaling pathways such as NDR1 and RAR1.<ref>PMID:20368970</ref> [[http://www.uniprot.org/uniprot/PBL2_ARATH PBL2_ARATH]] Involved in disease resistance signaling (PubMed:20413097, PubMed:23951354). Contributes to pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling and defense responses downstream of FLS2 (PubMed:20413097). Required for the bacterial XopAC/AvrAC effector-triggered immunity (ETI) against Xanthomonas campestris (PubMed:23951354).<ref>PMID:20413097</ref> <ref>PMID:23951354</ref>
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[https://www.uniprot.org/uniprot/R13L4_ARATH R13L4_ARATH] CC-NB-LRR receptor-like protein required for recognition of the Pseudomonas syringae type III effector HopZ1a. Confers allele-specific recognition and virulence attenuation of HopZ1a. Immunity mediated by ZAR1 is independent of several genes required by other resistance protein signaling pathways such as NDR1 and RAR1.<ref>PMID:20368970</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pathogen recognition by nucleotide-binding (NB), leucine-rich repeat (LRR) receptors (NLRs) plays roles in plant immunity. The Xanthomonas campestris pv. campestris effector AvrAC uridylylates the Arabidopsis PBL2 kinase, and the latter (PBL2(UMP)) acts as a ligand to activate the NLR ZAR1 precomplexed with the RKS1 pseudokinase. Here we report the cryo-electron microscopy structures of ZAR1-RKS1 and ZAR1-RKS1-PBL2(UMP) in an inactive and intermediate state, respectively. The ZAR1(LRR) domain, compared with animal NLR(LRR) domains, is differently positioned to sequester ZAR1 in an inactive state. Recognition of PBL2(UMP) is exclusively through RKS1, which interacts with ZAR1(LRR) PBL2(UMP) binding stabilizes the RKS1 activation segment, which sterically blocks ZAR1 adenosine diphosphate (ADP) binding. This engenders a more flexible NB domain without conformational changes in the other ZAR1 domains. Our study provides a structural template for understanding plant NLRs.
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Ligand-triggered allosteric ADP release primes a plant NLR complex.,Wang J, Wang J, Hu M, Wu S, Qi J, Wang G, Han Z, Qi Y, Gao N, Wang HW, Zhou JM, Chai J Science. 2019 Apr 5;364(6435). pii: 364/6435/eaav5868. doi:, 10.1126/science.aav5868. PMID:30948526<ref>PMID:30948526</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6j5u" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Non-specific serine/threonine protein kinase]]
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[[Category: Chai JJ]]
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[[Category: Hongwei, W]]
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[[Category: Meijuan H]]
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[[Category: Jianmin, Z]]
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[[Category: Wang HW]]
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[[Category: Jijie, C]]
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[[Category: Wang J]]
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[[Category: Jizong, W]]
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[[Category: Wang JZ]]
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[[Category: Meijuan, H]]
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[[Category: Zhou JM]]
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[[Category: Jia, W]]
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[[Category: Pbl2-ump]]
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[[Category: Plant protein]]
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[[Category: Rks1]]
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[[Category: Zar1]]
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Current revision

Ligand-triggered allosteric ADP release primes a plant NLR complex

6j5u, resolution 3.90Å

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