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| | <StructureSection load='6qsp' size='340' side='right'caption='[[6qsp]], [[Resolution|resolution]] 1.45Å' scene=''> | | <StructureSection load='6qsp' size='340' side='right'caption='[[6qsp]], [[Resolution|resolution]] 1.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6qsp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QSP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QSP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6qsp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenorhabdus_doucetiae Xenorhabdus doucetiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QSP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2iwz|2iwz]], [[1tqy|1tqy]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qsp OCA], [https://pdbe.org/6qsp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qsp RCSB], [https://www.ebi.ac.uk/pdbsum/6qsp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qsp ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qsp OCA], [http://pdbe.org/6qsp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qsp RCSB], [http://www.ebi.ac.uk/pdbsum/6qsp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qsp ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A068QVX9_9GAMM A0A068QVX9_9GAMM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bode, H]] | + | [[Category: Xenorhabdus doucetiae]] |
| - | [[Category: Grammbitter, G L.C]] | + | [[Category: Bode H]] |
| - | [[Category: Groll, M]] | + | [[Category: Grammbitter GLC]] |
| - | [[Category: Schmalhofer, M]] | + | [[Category: Groll M]] |
| - | [[Category: Aryl polyene pigment]] | + | [[Category: Schmalhofer M]] |
| - | [[Category: Biosynthesis]]
| + | |
| - | [[Category: Biosynthetic protein]]
| + | |
| - | [[Category: Chain elongation]]
| + | |
| - | [[Category: Claisen condensation]]
| + | |
| - | [[Category: Ketoacyl synthase]]
| + | |
| - | [[Category: Type ii polyketide synthase]]
| + | |
| Structural highlights
Function
A0A068QVX9_9GAMM
Publication Abstract from PubMed
Aryl polyene (APE) pigments are a widely distributed class of bacterial polyketides. So far, little is known about the biosynthesis of these compounds, which are produced by a novel type II polyketide synthase (PKS). We have identi-fied all enzymes involved in APE biosynthesis and determined their peculiar functions. The biosynthesis was recon-stituted in vitro and ACP-bound intermediates were assigned for each reaction step by HPLC-MS. Native mass spec-trometry experiments identified four stable complexes: the acyl-carrier proteins ApeE and ApeF bound to the thi-oesterase ApeK, the dehydratases ApeI and ApeP as well as the ketosynthase ApeO in complex with its chain-length factor ApeC. X-ray structures of the heterodimeric ApeO:ApeC and ApeI:ApeP complexes depict striking protein-protein interactions. Altogether, our study elucidated mechanistic aspects of APE biosynthesis that unifies elements of type II fatty acid and PKS systems, but in addition includes novel enzyme complexes.
An Uncommon Type II PKS Catalyzes Biosynthesis of Aryl Polyene Pigments.,Grammbitter GLC, Schmalhofer M, Karimi K, Schoner TA, Tobias NJ, Morgner N, Groll M, Bode HB J Am Chem Soc. 2019 Mar 25. doi: 10.1021/jacs.8b10776. PMID:30908039[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Grammbitter GLC, Schmalhofer M, Karimi K, Schoner TA, Tobias NJ, Morgner N, Groll M, Bode HB. An Uncommon Type II PKS Catalyzes Biosynthesis of Aryl Polyene Pigments. J Am Chem Soc. 2019 Mar 25. doi: 10.1021/jacs.8b10776. PMID:30908039 doi:http://dx.doi.org/10.1021/jacs.8b10776
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