|
|
| (One intermediate revision not shown.) |
| Line 3: |
Line 3: |
| | <StructureSection load='6grq' size='340' side='right'caption='[[6grq]], [[Resolution|resolution]] 3.30Å' scene=''> | | <StructureSection load='6grq' size='340' side='right'caption='[[6grq]], [[Resolution|resolution]] 3.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6grq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GRQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GRQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6grq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GRQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GRQ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Pirb, Lilrb3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6grq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6grq OCA], [http://pdbe.org/6grq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6grq RCSB], [http://www.ebi.ac.uk/pdbsum/6grq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6grq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6grq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6grq OCA], [https://pdbe.org/6grq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6grq RCSB], [https://www.ebi.ac.uk/pdbsum/6grq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6grq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8K4V6_MOUSE Q8K4V6_MOUSE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 17: |
Line 19: |
| | </div> | | </div> |
| | <div class="pdbe-citations 6grq" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6grq" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Leukocyte immunoglobulin-like receptor|Leukocyte immunoglobulin-like receptor]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| Line 22: |
Line 27: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Huizinga, E G]] | + | [[Category: Huizinga EG]] |
| - | [[Category: Janssen, B J.C]] | + | [[Category: Janssen BJC]] |
| - | [[Category: Vlieg, H C]] | + | [[Category: Vlieg HC]] |
| - | [[Category: B cell down regulation]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Neuronal growth inhibition]]
| + | |
| Structural highlights
Function
Q8K4V6_MOUSE
Publication Abstract from PubMed
Murine paired immunoglobulin receptor B (PirB) and its human ortholog leukocyte immunoglobulin-like receptor B2 (LILRB2) are widely expressed inhibitory receptors that interact with a diverse set of extracellular ligands and exert functions ranging from down-regulation of immune responses to inhibition of neuronal growth. However, structural information that could shed light on how PirB interacts with its ligands is lacking. Here, we report crystal structures of the PirB ectodomain; the first full ectodomain structure for a LILR family member, at 3.3-4.5 A resolution. The structures reveal that PirB's six Ig-like domains are arranged at acute angles, similar to the structures of leukocyte immunoglobulin-like receptor (LILR) and killer-cell immunoglobulin-like receptor (KIR). We observe that this regular arrangement is followed throughout the ectodomain, resulting in an extended zigzag conformation. In two out of the five structures reported here, the repeating zigzag is broken by the first domain that can adopt two alternative orientations. Quantitative binding experiments revealed a 9 mum dissociation constant for PirB-myelin-associated glycoprotein (MAG) ectodomain interactions. Taken together, these structural findings and the observed PirB-MAG interactions are compatible with a model for intercellular signaling in which the PirB extracellular domains, which point away from the cell surface, enable interaction with ligands in trans.
Structure and flexibility of the extracellular region of the PirB receptor.,Vlieg HC, Huizinga EG, Janssen BJC J Biol Chem. 2019 Mar 22;294(12):4634-4643. doi: 10.1074/jbc.RA118.004396. Epub, 2019 Jan 23. PMID:30674550[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Vlieg HC, Huizinga EG, Janssen BJC. Structure and flexibility of the extracellular region of the PirB receptor. J Biol Chem. 2019 Mar 22;294(12):4634-4643. doi: 10.1074/jbc.RA118.004396. Epub, 2019 Jan 23. PMID:30674550 doi:http://dx.doi.org/10.1074/jbc.RA118.004396
|
