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| | <StructureSection load='4mal' size='340' side='right'caption='[[4mal]], [[Resolution|resolution]] 2.05Å' scene=''> | | <StructureSection load='4mal' size='340' side='right'caption='[[4mal]], [[Resolution|resolution]] 2.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mal]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MAL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mal]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MAL FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fimV, PA3115 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mal OCA], [http://pdbe.org/4mal PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mal RCSB], [http://www.ebi.ac.uk/pdbsum/4mal PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mal ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mal OCA], [https://pdbe.org/4mal PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mal RCSB], [https://www.ebi.ac.uk/pdbsum/4mal PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mal ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/FIMV_PSEAE FIMV_PSEAE] Inner membrane hub protein that plays both cAMP-dependent and cAMP-independent roles in twitching motility (PubMed:27297880, PubMed:28583947). Regulates intracellular cyclic AMP (cAMP) levels through the activation of adenylate cyclase CyaB (PubMed:20345659). Plays an essential role in a number of virulence mechanisms including type IV pilus (T4P)-mediated assembly and twitching motility as well as cAMP-dependent virulence gene expression (PubMed:10846211, PubMed:21097635). Mediates also type II secretion (T2S) of lipases and proteases (PubMed:21527471). In addition, mediates the cAMP-independent localization of multiple T4P structural and regulatory components to the cell poles (PubMed:21527471, PubMed:27297880, PubMed:28583947). This role in directing proteins to the cell pole is not restricted to type IV component and involves other proteins such as the diguanylate cyclase DgcP (By similarity).[UniProtKB:A0A0H2ZC68]<ref>PMID:10846211</ref> <ref>PMID:20345659</ref> <ref>PMID:21097635</ref> <ref>PMID:21527471</ref> <ref>PMID:27297880</ref> <ref>PMID:28583947</ref> |
| | + | == References == |
| | + | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pseae]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Burrows, L L]] | + | [[Category: Burrows LL]] |
| - | [[Category: Daniel-Ivad, M]] | + | [[Category: Daniel-Ivad M]] |
| - | [[Category: Howell, P L]] | + | [[Category: Howell PL]] |
| - | [[Category: Junop, M S]] | + | [[Category: Junop MS]] |
| - | [[Category: Nguyen, Y]] | + | [[Category: Nguyen Y]] |
| - | [[Category: Sugiman-Marangos, S N]] | + | [[Category: Sugiman-Marangos SN]] |
| - | [[Category: Zhang, K]] | + | [[Category: Zhang K]] |
| - | [[Category: Tpr]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
FIMV_PSEAE Inner membrane hub protein that plays both cAMP-dependent and cAMP-independent roles in twitching motility (PubMed:27297880, PubMed:28583947). Regulates intracellular cyclic AMP (cAMP) levels through the activation of adenylate cyclase CyaB (PubMed:20345659). Plays an essential role in a number of virulence mechanisms including type IV pilus (T4P)-mediated assembly and twitching motility as well as cAMP-dependent virulence gene expression (PubMed:10846211, PubMed:21097635). Mediates also type II secretion (T2S) of lipases and proteases (PubMed:21527471). In addition, mediates the cAMP-independent localization of multiple T4P structural and regulatory components to the cell poles (PubMed:21527471, PubMed:27297880, PubMed:28583947). This role in directing proteins to the cell pole is not restricted to type IV component and involves other proteins such as the diguanylate cyclase DgcP (By similarity).[UniProtKB:A0A0H2ZC68][1] [2] [3] [4] [5] [6]
References
- ↑ Semmler AB, Whitchurch CB, Leech AJ, Mattick JS. Identification of a novel gene, fimV, involved in twitching motility in Pseudomonas aeruginosa. Microbiology. 2000 Jun;146 ( Pt 6):1321-32. PMID:10846211
- ↑ Fulcher NB, Holliday PM, Klem E, Cann MJ, Wolfgang MC. The Pseudomonas aeruginosa Chp chemosensory system regulates intracellular cAMP levels by modulating adenylate cyclase activity. Mol Microbiol. 2010 May;76(4):889-904. doi: 10.1111/j.1365-2958.2010.07135.x. , Epub 2010 Mar 16. PMID:20345659 doi:http://dx.doi.org/10.1111/j.1365-2958.2010.07135.x
- ↑ Wehbi H, Portillo E, Harvey H, Shimkoff AE, Scheurwater EM, Howell PL, Burrows LL. The peptidoglycan-binding protein FimV promotes assembly of the Pseudomonas aeruginosa type IV pilus secretin. J Bacteriol. 2011 Jan;193(2):540-50. doi: 10.1128/JB.01048-10. Epub 2010 Nov 19. PMID:21097635 doi:http://dx.doi.org/10.1128/JB.01048-10
- ↑ Michel GPF, Aguzzi A, Ball G, Soscia C, Bleves S, Voulhoux R. Role of fimV in type II secretion system-dependent protein secretion of Pseudomonas aeruginosa on solid medium. Microbiology (Reading). 2011 Jul;157(Pt 7):1945-1954. doi: , 10.1099/mic.0.045849-0. Epub 2011 Apr 28. PMID:21527471 doi:http://dx.doi.org/10.1099/mic.0.045849-0
- ↑ Buensuceso RN, Nguyen Y, Zhang K, Daniel-Ivad M, Sugiman-Marangos SN, Fleetwood AD, Zhulin IB, Junop MS, Howell PL, Burrows LL. The Conserved Tetratricopeptide Repeat-Containing C-Terminal Domain of Pseudomonas aeruginosa FimV Is Required for Its Cyclic AMP-Dependent and -Independent Functions. J Bacteriol. 2016 Jul 28;198(16):2263-74. doi: 10.1128/JB.00322-16. Print 2016 , Aug 15. PMID:27297880 doi:http://dx.doi.org/10.1128/JB.00322-16
- ↑ Buensuceso RNC, Daniel-Ivad M, Kilmury SLN, Leighton TL, Harvey H, Howell PL, Burrows LL. Cyclic AMP-Independent Control of Twitching Motility in Pseudomonas aeruginosa. J Bacteriol. 2017 Jul 25;199(16):e00188-17. doi: 10.1128/JB.00188-17. Print 2017 , Aug 15. PMID:28583947 doi:http://dx.doi.org/10.1128/JB.00188-17
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