4lug

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Crystal structure of Inorganic Pyrophosphatase PPA1 from Arabidopsis thaliana

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 <StructureSection load='4lug' size='340' side='right'caption='[[4lug]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4lug]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LUG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4lug]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LUG FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3emj|3emj]], [[3tr4|3tr4]], [[3i4q|3i4q]], [[1ygz|1ygz]], [[3fq3|3fq3]], [[1twl|1twl]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PPa1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lug OCA], [https://pdbe.org/4lug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lug RCSB], [https://www.ebi.ac.uk/pdbsum/4lug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lug ProSAT]</span></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lug OCA], [http://pdbe.org/4lug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lug RCSB], [http://www.ebi.ac.uk/pdbsum/4lug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lug ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/IPYR1_ARATH IPYR1_ARATH] Catalyzes the irreversible hydrolysis of pyrophosphate (PPi) to phosphate. The MgPPi(2-) complex binds to the enzyme only after a free Mg(2+) ion has bound (Ref.9). No activity with glycerol-3-phosphate, glucose-6-phosphate, p-nitrophenylphosphate, ADP, NADP(+), NAD(+),NADH, NADPH or phosphoribosyl pyrophosphate as substrates (Ref.9). Controls the equilibrium of gluconeogenic reactions in the heterotrophic growth phase of early seedling establishment. Determinates the rate of cytosolic glycolysis, providing carbon for seed storage lipid accumulation (PubMed:22566496).<ref>PMID:22566496</ref> [REFERENCE:9]
-An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl(2) was necessary for the protein's activity, whilst NaF inhibited it. The K(m) for pyrophosphate and the pH optimum of the protein was determined. The gene coding for this protein was expressed in all tissues, and its expression in rosette leaves was induced by incubation on metabolizable sugars. In vitro import experiments demonstrated that the protein could be imported into chloroplasts and localized to the stromal compartment.
-Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts.,Schulze S, Mant A, Kossmann J, Lloyd JR FEBS Lett. 2004 May 7;565(1-3):101-5. PMID:15135060<ref>PMID:15135060</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 4lug" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[Inorganic pyrophosphatase|Inorganic pyrophosphatase]]
+*[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Arath]]
+[[Category: Arabidopsis thaliana]]
-[[Category: Inorganic diphosphatase]]
 [[Category: Large Structures]]
-[[Category: Grzechowiak, M]]
+[[Category: Grzechowiak M]]
-[[Category: Jaskolski, M]]
+[[Category: Jaskolski M]]
-[[Category: Ruszkowski, M]]
+[[Category: Ruszkowski M]]
-[[Category: Sikorski, M]]
+[[Category: Sikorski M]]
-[[Category: Hydrolase]]
-[[Category: Hydrolysis of pyrophosphate]]
-[[Category: Magnesium ion binding]]
-[[Category: Metal binding]]

4lug

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Current revision

Crystal structure of Inorganic Pyrophosphatase PPA1 from Arabidopsis thaliana

Structural highlights

Function

See Also

References

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