2zbx

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[[Image:2zbx.jpg|left|200px]]
 
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==Crystal structure of vitamin D hydroxylase cytochrome P450 105A1 (wild type) with imidazole bound==
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The line below this paragraph, containing "STRUCTURE_2zbx", creates the "Structure Box" on the page.
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<StructureSection load='2zbx' size='340' side='right'caption='[[2zbx]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zbx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseolus Streptomyces griseolus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZBX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
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{{STRUCTURE_2zbx| PDB=2zbx | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zbx OCA], [https://pdbe.org/2zbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zbx RCSB], [https://www.ebi.ac.uk/pdbsum/2zbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zbx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CPXE_STRGO CPXE_STRGO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zb/2zbx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zbx ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of vitamin D hydroxylase cytochrome P450 105A1 (wild type) with imidazole bound'''
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==See Also==
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*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Vitamin D 3 (VD 3), a prohormone in mammals, plays a crucial role in the maintenance of calcium and phosphorus concentrations in serum. Activation of VD 3 requires 25-hydroxylation in the liver and 1alpha-hydroxylation in the kidney by cytochrome P450 (CYP) enzymes. Bacterial CYP105A1 converts VD 3 into 1alpha,25-dihydroxyvitamin D 3 (1alpha,25(OH) 2D 3) in two independent reactions, despite its low sequence identity with mammalian enzymes (&lt;21% identity). The present study determined the crystal structures of a highly active mutant (R84A) of CYP105A1 from Streptomyces griseolus in complex and not in complex with 1alpha,25(OH) 2D 3. The compound 1alpha,25(OH) 2D 3 is positioned 11 A from the iron atom along the I helix within the pocket. A similar binding mode is observed in the structure of the human CYP2R1-VD 3 complex, indicating a common substrate-binding mechanism for 25-hydroxylation. A comparison with the structure of wild-type CYP105A1 suggests that the loss of two hydrogen bonds in the R84A mutant increases the adaptability of the B' and F helices, creating a transient binding site. Further mutational analysis of the active site reveals that 25- and 1alpha-hydroxylations share residues that participate in these reactions. These results provide the structural basis for understanding the mechanism of the two-step hydroxylation that activates VD 3.
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[[Category: Large Structures]]
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==About this Structure==
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2ZBX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_griseolus Streptomyces griseolus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZBX OCA].
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==Reference==
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Crystal Structure of CYP105A1 (P450SU-1) in Complex with 1alpha,25-Dihydroxyvitamin D3(,)., Sugimoto H, Shinkyo R, Hayashi K, Yoneda S, Yamada M, Kamakura M, Ikushiro S, Shiro Y, Sakaki T, Biochemistry. 2008 Apr 1;47(13):4017-27. Epub 2008 Mar 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18314962 18314962]
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[[Category: Single protein]]
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[[Category: Streptomyces griseolus]]
[[Category: Streptomyces griseolus]]
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[[Category: Unspecific monooxygenase]]
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[[Category: Hayashi K]]
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[[Category: Hayashi, K.]]
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[[Category: Ikushiro S]]
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[[Category: Ikushiro, S.]]
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[[Category: Kamakura M]]
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[[Category: Kamakura, M.]]
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[[Category: Sakaki T]]
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[[Category: Sakaki, T.]]
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[[Category: Shinkyo R]]
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[[Category: Shinkyo, R.]]
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[[Category: Shiro Y]]
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[[Category: Shiro, Y.]]
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[[Category: Sugimoto H]]
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[[Category: Sugimoto, H.]]
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[[Category: Yamada M]]
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[[Category: Yamada, M.]]
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[[Category: Yoneda S]]
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[[Category: Yoneda, S.]]
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[[Category: Beta prism]]
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[[Category: Heme]]
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[[Category: Iron]]
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[[Category: Metal-binding]]
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[[Category: Monooxygenase]]
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[[Category: Oxidoreductase]]
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[[Category: P450]]
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Current revision

Crystal structure of vitamin D hydroxylase cytochrome P450 105A1 (wild type) with imidazole bound

PDB ID 2zbx

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