6r9v
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Pediococcus acidilactici lactate oxidase A94G mutant== | |
| + | <StructureSection load='6r9v' size='340' side='right'caption='[[6r9v]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6r9v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pediococcus_acidilactici_DSM_20284 Pediococcus acidilactici DSM 20284]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R9V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6R9V FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6r9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r9v OCA], [https://pdbe.org/6r9v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6r9v RCSB], [https://www.ebi.ac.uk/pdbsum/6r9v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6r9v ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/E0NE46_PEDAC E0NE46_PEDAC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lactate oxidases belong to a group of FMN-dependent enzymes and they catalyze a conversion of lactate to pyruvate with a release of hydrogen peroxide. Hydrogen peroxide is also utilized as a read out in biosensors to quantitate lactate levels in biological samples. Aerococcus viridans lactate oxidase is the best characterized lactate oxidase and our knowledge of lactate oxidases relies largely to studies conducted with that particular enzyme. Pediococcus acidilactici lactate oxidase is also commercially available for e.g. lactate measurements, but this enzyme has not been characterized in detail before. Here we report structural characterization of the recombinant enzyme and its co-factor dependent oligomerization. The crystal structures revealed two distinct conformations in the loop closing the active site, consistent with previous biochemical studies implicating the role of loop in catalysis. Despite the structural conservation of active site residues, we were not able to detect either oxidase or monooxygenase activity when L-lactate was used as a substrate. Pediococcus acidilactici lactate oxidase is therefore an example of a misannotation of an FMN-dependent enzyme, which catalyzes likely a so far unknown oxidation reaction. | ||
| - | + | FMN-dependent oligomerization of putative lactate oxidase from Pediococcus acidilactici.,Ashok Y, Maksimainen MM, Kallio T, Kilpelainen P, Lehtio L PLoS One. 2020 Feb 24;15(2):e0223870. doi: 10.1371/journal.pone.0223870., eCollection 2020. PMID:32092083<ref>PMID:32092083</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6r9v" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pediococcus acidilactici DSM 20284]] | ||
| + | [[Category: Ashok Y]] | ||
| + | [[Category: Lehtio L]] | ||
| + | [[Category: Maksimainen MM]] | ||
Current revision
Crystal structure of Pediococcus acidilactici lactate oxidase A94G mutant
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