5mhj

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<StructureSection load='5mhj' size='340' side='right'caption='[[5mhj]], [[Resolution|resolution]] 2.12&Aring;' scene=''>
<StructureSection load='5mhj' size='340' side='right'caption='[[5mhj]], [[Resolution|resolution]] 2.12&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5mhj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Hhv-1 Hhv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MHJ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5mhj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_alphaherpesvirus_1 Human alphaherpesvirus 1] and [https://en.wikipedia.org/wiki/Human_alphaherpesvirus_1_strain_17 Human alphaherpesvirus 1 strain 17]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MHJ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.117&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ICP4, IE175, RS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10299 HHV-1])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mhj OCA], [http://pdbe.org/5mhj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mhj RCSB], [http://www.ebi.ac.uk/pdbsum/5mhj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mhj ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mhj OCA], [https://pdbe.org/5mhj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mhj RCSB], [https://www.ebi.ac.uk/pdbsum/5mhj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mhj ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ICP4_HHV11 ICP4_HHV11]] Plays an essential role in the regulation of viral gene expression by both activating and repressing host RNA polymerase II-mediated transcription. Binds with high affinity to the sequence 5'-ATCGTC-3'. Activates transcription by recruiting a form of the host TFIID to promoters and stabilizing the pre-initiation complex formation. Negatively regulates its own transcription. This immediate early (IE) protein is absolutely necessary for the transition from IE transcription to later viral gene transcription.<ref>PMID:18094162</ref> <ref>PMID:18216093</ref> <ref>PMID:1846804</ref> <ref>PMID:22496239</ref> <ref>PMID:23135715</ref> <ref>PMID:24147125</ref> <ref>PMID:8649420</ref>
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[https://www.uniprot.org/uniprot/ICP4_HHV11 ICP4_HHV11] Plays an essential role in the regulation of viral gene expression by both activating and repressing host RNA polymerase II-mediated transcription. Binds with high affinity to the sequence 5'-ATCGTC-3'. Activates transcription by recruiting a form of the host TFIID to promoters and stabilizing the pre-initiation complex formation. Negatively regulates its own transcription. This immediate early (IE) protein is absolutely necessary for the transition from IE transcription to later viral gene transcription.<ref>PMID:18094162</ref> <ref>PMID:18216093</ref> <ref>PMID:1846804</ref> <ref>PMID:22496239</ref> <ref>PMID:23135715</ref> <ref>PMID:24147125</ref> <ref>PMID:8649420</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The transcription factor ICP4 from herpes simplex virus has a central role in regulating the gene expression cascade which controls viral infection. Here we present the crystal structure of the functionally essential ICP4 DNA binding domain in complex with a segment from its own promoter, revealing a novel homo-dimeric fold. We also studied the complex in solution by small angle X-Ray scattering, nuclear magnetic resonance and surface-plasmon resonance which indicated that, in addition to the globular domain, a flanking intrinsically disordered region also recognizes DNA. Together the data provides a rationale for the bi-partite nature of the ICP4 DNA recognition consensus sequence as the globular and disordered regions bind synergistically to adjacent DNA motifs. Therefore in common with its eukaryotic host, the viral transcription factor ICP4 utilizes disordered regions to enhance the affinity and tune the specificity of DNA interactions in tandem with a globular domain.
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The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.,Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP Nucleic Acids Res. 2017 May 13. doi: 10.1093/nar/gkx419. PMID:28505309<ref>PMID:28505309</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5mhj" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Hhv-1]]
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[[Category: Human alphaherpesvirus 1]]
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[[Category: Human alphaherpesvirus 1 strain 17]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Baldock, C]]
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[[Category: Baldock C]]
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[[Category: Golovanov, A P]]
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[[Category: Golovanov AP]]
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[[Category: Jowitt, T A]]
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[[Category: Jowitt TA]]
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[[Category: Levy, C]]
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[[Category: Levy C]]
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[[Category: Lockhart-Cairns, M P]]
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[[Category: Lockhart-Cairns MP]]
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[[Category: Mould, P]]
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[[Category: Mould P]]
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[[Category: Sandri-Goldin, R M]]
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[[Category: Sandri-Goldin RM]]
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[[Category: Sito, H]]
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[[Category: Sito H]]
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[[Category: Tunnicliffe, R B]]
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[[Category: Tunnicliffe RB]]
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[[Category: Dimer]]
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[[Category: Herpes]]
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[[Category: Transcription]]
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[[Category: Transcription factor]]
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[[Category: Virus]]
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Current revision

ICP4 DNA-binding domain, lacking intrinsically disordered region, in complex with 12mer DNA duplex from its own promoter

PDB ID 5mhj

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