|
|
| (One intermediate revision not shown.) |
| Line 3: |
Line 3: |
| | <StructureSection load='4n0t' size='340' side='right'caption='[[4n0t]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='4n0t' size='340' side='right'caption='[[4n0t]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4n0t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N0T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4n0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N0T FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRP24, YM8156.10C, YMR268C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n0t OCA], [http://pdbe.org/4n0t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n0t RCSB], [http://www.ebi.ac.uk/pdbsum/4n0t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n0t ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n0t OCA], [https://pdbe.org/4n0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n0t RCSB], [https://www.ebi.ac.uk/pdbsum/4n0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n0t ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PRP24_YEAST PRP24_YEAST]] Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing. | + | [https://www.uniprot.org/uniprot/PRP24_YEAST PRP24_YEAST] Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Andrews, K L]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Brow, D A]] | + | [[Category: Andrews KL]] |
| - | [[Category: Butcher, S E]] | + | [[Category: Brow DA]] |
| - | [[Category: Curran, E C]] | + | [[Category: Butcher SE]] |
| - | [[Category: Liao, H]] | + | [[Category: Curran EC]] |
| - | [[Category: Montemayor, E J]] | + | [[Category: Liao H]] |
| - | [[Category: Treba, C N]] | + | [[Category: Montemayor EJ]] |
| - | [[Category: Annealing u6 and u4 snrna]]
| + | [[Category: Treba CN]] |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Rna binding protein-rna complex]]
| + | |
| - | [[Category: Spliceosomal ribonucleoprotein complex]]
| + | |
| Structural highlights
Function
PRP24_YEAST Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.
Publication Abstract from PubMed
The spliceosome is a dynamic assembly of five small nuclear ribonucleoproteins (snRNPs) that removes introns from eukaryotic pre-mRNA. U6, the most conserved of the spliceosomal small nuclear RNAs (snRNAs), participates directly in catalysis. Here, we report the crystal structure of the Saccharomyces cerevisiae U6 snRNP core containing most of the U6 snRNA and all four RRM domains of the Prp24 protein. It reveals a unique interlocked RNP architecture that sequesters the 5' splice site-binding bases of U6 snRNA. RRMs 1, 2 and 4 of Prp24 form an electropositive groove that binds double-stranded RNA and may nucleate annealing of U4 and U6 snRNAs. Substitutions in Prp24 that suppress a mutation in U6 localize to direct RNA-protein contacts. Our results provide the most comprehensive view to date of a multi-RRM protein bound to RNA and reveal striking coevolution of protein and RNA structure.
Core structure of the U6 small nuclear ribonucleoprotein at 1.7-A resolution.,Montemayor EJ, Curran EC, Liao HH, Andrews KL, Treba CN, Butcher SE, Brow DA Nat Struct Mol Biol. 2014 Jun;21(6):544-51. doi: 10.1038/nsmb.2832. Epub 2014 May, 18. PMID:24837192[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Montemayor EJ, Curran EC, Liao HH, Andrews KL, Treba CN, Butcher SE, Brow DA. Core structure of the U6 small nuclear ribonucleoprotein at 1.7-A resolution. Nat Struct Mol Biol. 2014 Jun;21(6):544-51. doi: 10.1038/nsmb.2832. Epub 2014 May, 18. PMID:24837192 doi:http://dx.doi.org/10.1038/nsmb.2832
|
