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| | <StructureSection load='4mhu' size='340' side='right'caption='[[4mhu]], [[Resolution|resolution]] 2.56Å' scene=''> | | <StructureSection load='4mhu' size='340' side='right'caption='[[4mhu]], [[Resolution|resolution]] 2.56Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mhu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphal Sphal]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MHU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mhu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingopyxis_alaskensis_RB2256 Sphingopyxis alaskensis RB2256]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MHU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MHU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=D9G:N-DODECYL-N,N-DIMETHYLGLYCINATE'>D9G</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.56Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3emr|3emr]], [[4mhr|4mhr]], [[4q5o|4q5o]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D9G:N-DODECYL-N,N-DIMETHYLGLYCINATE'>D9G</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Sala_2952 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=317655 SPHAL])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mhu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mhu OCA], [https://pdbe.org/4mhu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mhu RCSB], [https://www.ebi.ac.uk/pdbsum/4mhu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mhu ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mhu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mhu OCA], [http://pdbe.org/4mhu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mhu RCSB], [http://www.ebi.ac.uk/pdbsum/4mhu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mhu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/ECTD_SPHAL ECTD_SPHAL] Involved in the biosynthesis of 5-hydroxyectoine, called compatible solute, which helps organisms to survive extreme osmotic stress by acting as a highly soluble organic osmolyte. Catalyzes the 2-oxoglutarate-dependent selective hydroxylation of L-ectoine to yield (4S,5S)-5-hydroxyectoine.<ref>PMID:24714029</ref> <ref>PMID:25172507</ref> |
| - | Ectoine and its derivative 5-hydroxyectoine are compatible solutes that are widely synthesized by Bacteria to cope physiologically with osmotic stress. They also serve as chemical chaperones and maintain the functionality of macromolecules. 5-hydroxyectoine is produced from ectoine through a stereo-specific hydroxylation, an enzymatic reaction catalyzed by the ectoine hydroxylase (EctD). The EctD protein is a member of the non-heme-containing iron (II) and 2-oxoglutarate-dependent dioxygenase superfamily and is evolutionarily well conserved. We studied the ectoine hydroxylase from the cold-adapted marine ultra-microbacterium Sphingopyxis alaskensis (Sa) and found that the purified SaEctD protein is a homo-dimer in solution. We determined the SaEctD crystal structure in its apo-form, complexed with the iron catalyst and in a form that contained iron, the co-substrate 2-oxoglutarate, and the reaction product of EctD, 5-hydroxyectoine. The iron and 2-oxoglutarate ligands are bound within the EctD active site in a fashion similar to that found in other members of the dioxygenase superfamily. 5-hydroxyectoine on the other hand, is coordinated by EctD in manner different from that found in high-affinity solute receptor proteins operating in conjunction with microbial import systems for ectoines. Our crystallographic analysis provides a detailed view into the active site of the ectoine hydroxylase and exposes an intricate network of interactions between the enzyme and its ligands that collectively ensure the hydroxylation of the ectoine substrate in a position- and stereo-specific manner.
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| - | Crystal Structure of the Ectoine Hydroxylase: a Snapshot of the Active Site.,Hoeppner A, Widderich N, Lenders M, Bremer E, Smits SH J Biol Chem. 2014 Aug 29. pii: jbc.M114.576769. PMID:25172507<ref>PMID:25172507</ref>
| + | ==See Also== |
| - | | + | *[[Hydroxylases 3D structures|Hydroxylases 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 4mhu" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sphal]] | + | [[Category: Sphingopyxis alaskensis RB2256]] |
| - | [[Category: Bremer, E]] | + | [[Category: Bremer E]] |
| - | [[Category: Heider, J]] | + | [[Category: Heider J]] |
| - | [[Category: Hoeppner, A]] | + | [[Category: Hoeppner A]] |
| - | [[Category: Pittelkow, M]] | + | [[Category: Pittelkow M]] |
| - | [[Category: Smits, S H]] | + | [[Category: Smits SH]] |
| - | [[Category: Widderich, N]] | + | [[Category: Widderich N]] |
| - | [[Category: Ectoine]]
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| - | [[Category: Ectoine hydroxylase]]
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| - | [[Category: Jelly-roll or cupin fold]]
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| - | [[Category: Oxidoreductase]]
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