This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3bzk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px <!-- The line below this paragraph, containing "STRUCTURE_3bzk", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...)
Current revision (12:18, 30 August 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3bzk.jpg|left|200px]]
 
-
<!--
+
==Crystal Structure of the Tex protein from Pseudomonas aeruginosa, crystal form 2==
-
The line below this paragraph, containing "STRUCTURE_3bzk", creates the "Structure Box" on the page.
+
<StructureSection load='3bzk' size='340' side='right'caption='[[3bzk]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3bzk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BZK FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bzk OCA], [https://pdbe.org/3bzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bzk RCSB], [https://www.ebi.ac.uk/pdbsum/3bzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bzk ProSAT]</span></td></tr>
-
{{STRUCTURE_3bzk| PDB=3bzk | SCENE= }}
+
</table>
-
 
+
== Function ==
-
'''Crystal Structure of the Tex protein from Pseudomonas aeruginosa, crystal form 2'''
+
[https://www.uniprot.org/uniprot/Q9HTY8_PSEAE Q9HTY8_PSEAE]
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/3bzk_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bzk ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
Tex is a highly conserved bacterial protein that likely functions in a variety of transcriptional processes. Here, we describe two crystal structures of the 86-kDa Tex protein from Pseudomonas aeruginosa at 2.3 and 2.5 A resolution, respectively. These structures reveal a relatively flat and elongated protein, with several potential nucleic acid binding motifs clustered at one end, including an S1 domain near the C-terminus that displays considerable structural flexibility. Tex binds nucleic acids, with a preference for single-stranded RNA, and the Tex S1 domain is required for this binding activity. Point mutants further demonstrate that the primary nucleic acid binding site corresponds to a surface of the S1 domain. Sequence alignment and modeling indicate that the eukaryotic Spt6 transcription factor adopts a similar core structure. Structural analysis further suggests that the RNA polymerase and nucleosome interacting regions of Spt6 flank opposite sides of the Tex-like scaffold. Therefore, the Tex structure may represent a conserved scaffold that binds single-stranded RNA to regulate transcription in both eukaryotic and prokaryotic organisms.
Tex is a highly conserved bacterial protein that likely functions in a variety of transcriptional processes. Here, we describe two crystal structures of the 86-kDa Tex protein from Pseudomonas aeruginosa at 2.3 and 2.5 A resolution, respectively. These structures reveal a relatively flat and elongated protein, with several potential nucleic acid binding motifs clustered at one end, including an S1 domain near the C-terminus that displays considerable structural flexibility. Tex binds nucleic acids, with a preference for single-stranded RNA, and the Tex S1 domain is required for this binding activity. Point mutants further demonstrate that the primary nucleic acid binding site corresponds to a surface of the S1 domain. Sequence alignment and modeling indicate that the eukaryotic Spt6 transcription factor adopts a similar core structure. Structural analysis further suggests that the RNA polymerase and nucleosome interacting regions of Spt6 flank opposite sides of the Tex-like scaffold. Therefore, the Tex structure may represent a conserved scaffold that binds single-stranded RNA to regulate transcription in both eukaryotic and prokaryotic organisms.
-
==About this Structure==
+
Crystal structure and RNA binding of the Tex protein from Pseudomonas aeruginosa.,Johnson SJ, Close D, Robinson H, Vallet-Gely I, Dove SL, Hill CP J Mol Biol. 2008 Apr 11;377(5):1460-73. Epub 2008 Feb 12. PMID:18321528<ref>PMID:18321528</ref>
-
3BZK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BZK OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structure and RNA binding of the Tex protein from Pseudomonas aeruginosa., Johnson SJ, Close D, Robinson H, Vallet-Gely I, Dove SL, Hill CP, J Mol Biol. 2008 Apr 11;377(5):1460-73. Epub 2008 Feb 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18321528 18321528]
+
</div>
 +
<div class="pdbe-citations 3bzk" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
-
[[Category: Single protein]]
+
[[Category: Close D]]
-
[[Category: Close, D.]]
+
[[Category: Hill CP]]
-
[[Category: Hill, C P.]]
+
[[Category: Johnson SJ]]
-
[[Category: Johnson, S J.]]
+
-
[[Category: Helix-hairpin-helix]]
+
-
[[Category: Helix-turn-helix]]
+
-
[[Category: Rna binding protein]]
+
-
[[Category: S1 domain]]
+
-
[[Category: Transcription]]
+
-
[[Category: Yqgf domain]]
+

Current revision

Crystal Structure of the Tex protein from Pseudomonas aeruginosa, crystal form 2

PDB ID 3bzk

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bzk"
Personal tools