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| | <StructureSection load='6jmi' size='340' side='right'caption='[[6jmi]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='6jmi' size='340' side='right'caption='[[6jmi]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6jmi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5xpq 5xpq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JMI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JMI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6jmi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JMI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JMI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.896Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5xpq|5xpq]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rv0081 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jmi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jmi OCA], [https://pdbe.org/6jmi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jmi RCSB], [https://www.ebi.ac.uk/pdbsum/6jmi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jmi ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jmi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jmi OCA], [http://pdbe.org/6jmi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jmi RCSB], [http://www.ebi.ac.uk/pdbsum/6jmi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jmi ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Y0081_MYCTU Y0081_MYCTU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Myctu]] | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Kumar, A]] | + | [[Category: Kumar A]] |
| - | [[Category: Mande, C S]] | + | [[Category: Mande CS]] |
| - | [[Category: Phulera, S]] | + | [[Category: Phulera S]] |
| - | [[Category: Arsr/smtb]]
| + | |
| - | [[Category: Fhl regulator]]
| + | |
| - | [[Category: Hypoxia]]
| + | |
| - | [[Category: Regulatory hub]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
Y0081_MYCTU
Publication Abstract from PubMed
The transcription factor Rv0081 of Mycobacterium tuberculosis controls hypoxic gene expression and acts as a regulatory hub in the latent phase of tuberculosis infection. We report here the crystal structure of Rv0081 at 2.9 A resolution revealing that it belongs to the well-known ArsR/SmtB family proteins. However, unlike other members in this family, Rv0081 has neither a metal binding domain nor does it possess Cys residues, suggesting an alternate mechanism of gene regulation. Our structural and biochemical analyses suggest the molecular basis for the recognition of self-regulatory DNA sequences and a plausible mechanism of regulation of Rv0081 in the latent phase of tuberculosis infection. This article is protected by copyright. All rights reserved.
Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of Mycobacterium tuberculosis.,Kumar A, Phulera S, Rizvi A, Sonawane PJ, Panwar HS, Banerjee S, Sahu A, Mande SC FEBS Lett. 2019 Apr 2. doi: 10.1002/1873-3468.13375. PMID:30941756[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kumar A, Phulera S, Rizvi A, Sonawane PJ, Panwar HS, Banerjee S, Sahu A, Mande SC. Structural basis of hypoxic gene regulation by the Rv0081 transcription factor of Mycobacterium tuberculosis. FEBS Lett. 2019 Apr 2. doi: 10.1002/1873-3468.13375. PMID:30941756 doi:http://dx.doi.org/10.1002/1873-3468.13375
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