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| | <StructureSection load='4oiy' size='340' side='right'caption='[[4oiy]], [[Resolution|resolution]] 1.50Å' scene=''> | | <StructureSection load='4oiy' size='340' side='right'caption='[[4oiy]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4oiy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OIY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OIY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4oiy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OIY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OIY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SEC7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oiy OCA], [http://pdbe.org/4oiy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oiy RCSB], [http://www.ebi.ac.uk/pdbsum/4oiy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oiy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oiy OCA], [https://pdbe.org/4oiy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oiy RCSB], [https://www.ebi.ac.uk/pdbsum/4oiy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oiy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SEC7_YEAST SEC7_YEAST]] May play a role in vesicular budding and traffic between compartments of the Golgi apparatus. | + | [https://www.uniprot.org/uniprot/SEC7_YEAST SEC7_YEAST] May play a role in vesicular budding and traffic between compartments of the Golgi apparatus. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Qiu, B]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Sun, F]] | + | [[Category: Qiu B]] |
| - | [[Category: Zhang, K]] | + | [[Category: Sun F]] |
| - | [[Category: Guanine nucleotide exchange protein for arf]] | + | [[Category: Zhang K]] |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Sec7 domain]]
| + | |
| Structural highlights
Function
SEC7_YEAST May play a role in vesicular budding and traffic between compartments of the Golgi apparatus.
Publication Abstract from PubMed
ADP-ribosylation factors (Arfs) play key roles in controlling membrane traffic and organelle structures. The activation of Arfs from GDP to GTP binding form is triggered by the guanine exchange factors (GEFs). There are six families of Arf-GEFs with a common guanine exchange catalytic domain (Sec7 domain) and various mechanisms of guanine exchange activity regulation. A loop region (loop>J motif) just following the helix J of Sec7 domain was found conserved and important for the catalytic activity regulation of Arf-GEFs. However, the molecular detail of the role the loop>J motif plays has been yet unclear. Here, we studied the catalytic domain of Sec7p, a yeast trans-Golgi network membrane localized Arf-GEFs, and found that the loop>J motif is indispensible for its GEF catalytic activity. Crystallographic, NMR spectrum and mutagenesis studies suggested that the loop>J motif with a key conserved residue Ile1010 modulates the fine conformation of Sec7 domain and thereby regulates its guanine exchange activity.
C-terminal motif within Sec7 domain regulates guanine nucleotide exchange activity via tuning protein conformation.,Qiu B, Zhang K, Wang S, Sun F Biochem Biophys Res Commun. 2014 Mar 28;446(1):380-6. doi:, 10.1016/j.bbrc.2014.02.125. Epub 2014 Mar 5. PMID:24613384[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Qiu B, Zhang K, Wang S, Sun F. C-terminal motif within Sec7 domain regulates guanine nucleotide exchange activity via tuning protein conformation. Biochem Biophys Res Commun. 2014 Mar 28;446(1):380-6. doi:, 10.1016/j.bbrc.2014.02.125. Epub 2014 Mar 5. PMID:24613384 doi:http://dx.doi.org/10.1016/j.bbrc.2014.02.125
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