4otv

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<StructureSection load='4otv' size='340' side='right'caption='[[4otv]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
<StructureSection load='4otv' size='340' side='right'caption='[[4otv]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4otv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Operophtera_brumata_cypovirus_18 Operophtera brumata cypovirus 18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OTV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OTV FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4otv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Operophtera_brumata_cypovirus_18 Operophtera brumata cypovirus 18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OTV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ots|4ots]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4otv OCA], [http://pdbe.org/4otv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4otv RCSB], [http://www.ebi.ac.uk/pdbsum/4otv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4otv ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4otv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4otv OCA], [https://pdbe.org/4otv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4otv RCSB], [https://www.ebi.ac.uk/pdbsum/4otv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4otv ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q30C70_9VIRU Q30C70_9VIRU]
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This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of in cellulo and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.
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In cellulo structure determination of a novel cypovirus polyhedrin.,Axford D, Ji X, Stuart DI, Sutton G Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1435-41. doi:, 10.1107/S1399004714004714. Epub 2014 Apr 30. PMID:24816111<ref>PMID:24816111</ref>
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==See Also==
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*[[Polyhedrin|Polyhedrin]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4otv" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Operophtera brumata cypovirus 18]]
[[Category: Operophtera brumata cypovirus 18]]
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[[Category: Axford, D]]
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[[Category: Axford D]]
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[[Category: Ji, X]]
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[[Category: Ji X]]
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[[Category: Stuart, D I]]
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[[Category: Stuart DI]]
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[[Category: Sutton, G C]]
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[[Category: Sutton GC]]
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[[Category: Microcrystal]]
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[[Category: Natural crystal to protect virus]]
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[[Category: Occlusion body]]
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[[Category: Polyhedra]]
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[[Category: Viral protein]]
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Current revision

Crystal structure of in cellulo Operophtera brumata CPV18

PDB ID 4otv

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