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| | <StructureSection load='4p27' size='340' side='right'caption='[[4p27]], [[Resolution|resolution]] 2.16Å' scene=''> | | <StructureSection load='4p27' size='340' side='right'caption='[[4p27]], [[Resolution|resolution]] 2.16Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4p27]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P27 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4p27]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_mansoni Schistosoma mansoni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P27 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Smp_002070 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6183 Blood fluke])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p27 OCA], [http://pdbe.org/4p27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p27 RCSB], [http://www.ebi.ac.uk/pdbsum/4p27 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p27 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p27 OCA], [https://pdbe.org/4p27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p27 RCSB], [https://www.ebi.ac.uk/pdbsum/4p27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p27 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q1X6L4_SCHMA Q1X6L4_SCHMA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Blood fluke]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Asojo, O A]] | + | [[Category: Schistosoma mansoni]] |
| - | [[Category: Allergen]] | + | [[Category: Asojo OA]] |
| - | [[Category: Pathogenesis related protein]]
| + | |
| - | [[Category: Scp/tap]]
| + | |
| Structural highlights
Function
Q1X6L4_SCHMA
Publication Abstract from PubMed
Schistosomiasis is a parasitic disease that affects over 200 million people. Vaccine candidates have been identified, including Schistosoma mansoni venom allergen-like proteins (SmVALs) from the SCP/TAPS (sperm-coating protein/Tpx/antigen 5/pathogenesis related-1/Sc7) superfamily. The first SmVAL structure, SmVAL4, was refined to a resolution limit of 2.16 A. SmVAL4 has a unique structure that could not be predicted from homologous structures, with longer loops and an unusual C-terminal extension. SmVAL4 has the characteristic alpha/beta-sandwich and central SCP/TAPS cavity. Furthermore, SmVAL4 has only one of the signature CAP cavity tetrad amino-acid residues and is missing the histidines that coordinate divalent cations such as Zn(2+) in other SCP/TAPS proteins. SmVAL4 has a cavity between alpha-helices 1 and 4 that was observed to bind lipids in tablysin-15, suggesting the ability to bind lipids. Subsequently, SmVAL4 was shown to bind cholesterol in vitro. Additionally, SmVAL4 was shown to complement the in vivo sterol-export phenotype of yeast mutants lacking their endogenous CAP proteins. Expression of SmVAL4 in yeast cells lacking endogenous CAP function restores the block in sterol export. These studies suggest an evolutionarily conserved lipid-binding function shared by CAP proteins such as SmVAL4 and yeast CAP proteins such as Pry1.
Schistosoma mansoni venom allergen-like protein 4 (SmVAL4) is a novel lipid-binding SCP/TAPS protein that lacks the prototypical CAP motifs.,Kelleher A, Darwiche R, Rezende WC, Farias LP, Leite LC, Schneiter R, Asojo OA Acta Crystallogr D Biol Crystallogr. 2014 Aug;70(Pt 8):2186-96. doi:, 10.1107/S1399004714013315. Epub 2014 Jul 25. PMID:25084337[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kelleher A, Darwiche R, Rezende WC, Farias LP, Leite LC, Schneiter R, Asojo OA. Schistosoma mansoni venom allergen-like protein 4 (SmVAL4) is a novel lipid-binding SCP/TAPS protein that lacks the prototypical CAP motifs. Acta Crystallogr D Biol Crystallogr. 2014 Aug;70(Pt 8):2186-96. doi:, 10.1107/S1399004714013315. Epub 2014 Jul 25. PMID:25084337 doi:http://dx.doi.org/10.1107/S1399004714013315
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