6rej

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'''Unreleased structure'''
 
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The entry 6rej is ON HOLD
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==Crystal structure of Pizza6-SH with Zn2+==
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<StructureSection load='6rej' size='340' side='right'caption='[[6rej]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6rej]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6REJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6REJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rej OCA], [https://pdbe.org/6rej PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rej RCSB], [https://www.ebi.ac.uk/pdbsum/6rej PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rej ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We developed an artificial hydrolase based on the symmetrical Pizza6 beta-propeller protein for the metal-free hydrolysis of 4-nitrophenyl acetate and butyrate. Through site-specific mutagenesis and crystallisation studies, the catalytic mechanism was investigated and found to be dependent on a threonine-histidine dyad. The mutant with additional histidine residues generated the highest kcat values, forming a His-His-Thr triad and matched previously reported metalloenzymes. The highly symmetrical beta-propeller artificial enzymes and their protein-metal complexes have potential to be utilised in bioinorganic and supramolecular chemistry, as well as being developed further into 2D/3D catalytic materials.
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Authors: Noguchi, H., Clarke, D.E., Gryspeerdt, J.L., Feyter, S.D., Voet, A.R.D.
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Artificial beta-propeller protein-based hydrolases.,Clarke DE, Noguchi H, Gryspeerdt JAG, De Feyter S, Voet ARD Chem Commun (Camb). 2019 Jul 23;55(60):8880-8883. doi: 10.1039/c9cc04388h. PMID:31321399<ref>PMID:31321399</ref>
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Description: Crystal structure of Pizza6-SH with Zn2+
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Noguchi, H]]
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<div class="pdbe-citations 6rej" style="background-color:#fffaf0;"></div>
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[[Category: Clarke, D.E]]
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== References ==
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[[Category: Voet, A.R.D]]
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<references/>
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[[Category: Gryspeerdt, J.L]]
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__TOC__
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[[Category: Feyter, S.D]]
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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[[Category: Clarke DE]]
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[[Category: Feyter SD]]
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[[Category: Gryspeerdt JL]]
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[[Category: Noguchi H]]
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[[Category: Voet ARD]]

Current revision

Crystal structure of Pizza6-SH with Zn2+

PDB ID 6rej

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