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6rfm
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6rfm is ON HOLD Authors: Description: Category: Unreleased Structures) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Bordetella pertussis adenylate cyclase toxin transmembrane segment 411-490 in DPC micelles== | |
| + | <StructureSection load='6rfm' size='340' side='right'caption='[[6rfm]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6rfm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_pertussis Bordetella pertussis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RFM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RFM FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rfm OCA], [https://pdbe.org/6rfm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rfm RCSB], [https://www.ebi.ac.uk/pdbsum/6rfm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rfm ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CYAA_BORPE CYAA_BORPE] This adenylate cyclase belongs to a special class of bacterial toxin. It causes whooping cough by acting on mammalian cells by elevating cAMP-concentration and thus disrupts normal cell function. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Two distinct conformers of the adenylate cyclase toxin (CyaA) appear to accomplish its two parallel activities within target cell membrane. The translocating conformer would deliver the N-terminal adenylyl cyclase (AC) enzyme domain across plasma membrane into cytosol of cells, while the pore precursor conformer would assemble into oligomeric cation-selective pores and permeabilize cellular membrane. Both toxin activities then involve a membrane-interacting 'AC-to-Hly-linking segment' (residues 400 to 500). Here, we report the NMR structure of the corresponding CyaA411-490 polypeptide in dodecylphosphocholine micelles and show that it consists of two alpha-helices linked by an unrestrained loop. The N-terminal alpha-helix (Gly418 to His439) remained solvent accessible, while the C-terminal alpha-helix (His457 to Phe485) was fully enclosed within detergent micelles. CyaA411-490 weakly bound Ca(2+) ions (apparent KD 2.6 mM) and permeabilized negatively charged lipid vesicles. At high concentrations (10 muM) the CyaA411-490 polypeptide formed stable conductance units in artificial lipid bilayers with applied voltage, suggesting its possible transmembrane orientation in the membrane-inserted toxin. Mutagenesis revealed that two clusters of negatively charged residues within the 'AC-to-Hly-linking segment' (Glu419 to Glu432 and Asp445 to Glu448) regulate the balance between the AC domain translocating and pore-forming capacities of CyaA in function of calcium concentration. | ||
| - | + | Negative charge of the AC-to-Hly linking segment modulates calcium-dependent membrane activities of Bordetella adenylate cyclase toxin.,Sukova A, Bumba L, Srb P, Veverka V, Stanek O, Holubova J, Chmelik J, Fiser R, Sebo P, Masin J Biochim Biophys Acta Biomembr. 2020 Sep 1;1862(9):183310. doi:, 10.1016/j.bbamem.2020.183310. Epub 2020 Apr 22. PMID:32333856<ref>PMID:32333856</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6rfm" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bordetella pertussis]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bumba L]] | ||
| + | [[Category: Masin J]] | ||
| + | [[Category: Veverka V]] | ||
Current revision
Bordetella pertussis adenylate cyclase toxin transmembrane segment 411-490 in DPC micelles
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