6o19
From Proteopedia
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<StructureSection load='6o19' size='340' side='right'caption='[[6o19]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='6o19' size='340' side='right'caption='[[6o19]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6o19]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O19 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6o19]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O19 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.596Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o19 OCA], [https://pdbe.org/6o19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o19 RCSB], [https://www.ebi.ac.uk/pdbsum/6o19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o19 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/YBCB_SCHPO YBCB_SCHPO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pho7 is the fission yeast Zn2Cys6 transcriptional factor that drives a response to phosphate starvation in which phosphate acquisition genes are upregulated. Here we report a crystal structure at 1.6 A resolution of the Pho7 DNA-binding domain (DBD) bound at its target site 2 in the pho1 promoter (5'-TCGGAAATTAAAAA). Comparison to the previously reported structure of Pho7 DBD in complex with its binding site in the tgp1 promoter (5'-TCGGACATTCAAAT) reveals shared determinants of target site specificity as well as variations in the protein-DNA interface that accommodate different promoter DNA sequences. Mutagenesis of Pho7 amino acids at the DNA interface identified nucleobase contacts at the periphery of the footprint that are essential for the induction of pho1 expression in response to phosphate starvation and for Pho7 binding to site 1 in the pho1 promoter. | ||
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| + | Structure of fission yeast transcription factor Pho7 bound to pho1 promoter DNA and effect of Pho7 mutations on DNA binding and phosphate homeostasis.,Garg A, Goldgur Y, Sanchez AM, Schwer B, Shuman S Mol Cell Biol. 2019 Apr 22. pii: MCB.00132-19. doi: 10.1128/MCB.00132-19. PMID:31010807<ref>PMID:31010807</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6o19" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Schizosaccharomyces pombe]] |
| - | [[Category: | + | [[Category: Garg A]] |
| - | [[Category: | + | [[Category: Goldgur Y]] |
| - | [[Category: | + | [[Category: Shuman S]] |
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Current revision
Crystal Structure of Pho7 complex with pho1 promoter site 2
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