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4r0o

From Proteopedia

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Crystal structure of PEGylated plastocyanin at 4.2 A resolution

Structural highlights

4r0o is a 4 chain structure with sequence from Phormidium laminosum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 4.2Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PLAS_PHOLA Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.

Publication Abstract from PubMed

PEGylated proteins are a mainstay of the biopharmaceutical industry. Although the use of poly(ethylene glycol) (PEG) to increase particle size, stability and solubility is well-established, questions remain as to the structure of PEG-protein conjugates. Here we report the structural characterization of a model beta-sheet protein (plastocyanin, 11.5 kDa) modified with a single PEG 5,000. An NMR spectroscopy study of the PEGylated conjugate indicated that the protein and PEG behaved as independent domains. A crystal structure revealed an extraordinary double-helical assembly of the conjugate, with the helices arranged orthogonally to yield a highly porous architecture. Electron density was not observed for the PEG chain, which indicates that it was disordered. The volume available per PEG chain in the crystal was within 10% of the calculated random coil volume. Together, these data support a minimal interaction between the protein and the synthetic polymer. Our work provides new possibilities for understanding this important class of protein-polymer hybrids and suggests a novel approach to engineering protein assemblies.

Structure of a PEGylated protein reveals a highly porous double-helical assembly.,Cattani G, Vogeley L, Crowley PB Nat Chem. 2015 Oct;7(10):823-8. doi: 10.1038/nchem.2342. Epub 2015 Sep 7. PMID:26391082^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cattani G, Vogeley L, Crowley PB. Structure of a PEGylated protein reveals a highly porous double-helical assembly. Nat Chem. 2015 Oct;7(10):823-8. doi: 10.1038/nchem.2342. Epub 2015 Sep 7. PMID:26391082 doi:http://dx.doi.org/10.1038/nchem.2342

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4r0o"

Categories: Large Structures | Phormidium laminosum | Cattani G | Crowley PB | Vogeley L

@@ Line 3: / Line 3: @@
 <StructureSection load='4r0o' size='340' side='right'caption='[[4r0o]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4r0o]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Phola Phola]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R0O FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4r0o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Phormidium_laminosum Phormidium laminosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R0O FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=LCY:1-METHYLPYRROLIDINE-2,5-DIONE'>LCY</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">petE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32059 PHOLA])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=LCY:1-METHYLPYRROLIDINE-2,5-DIONE'>LCY</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0o OCA], [http://pdbe.org/4r0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r0o RCSB], [http://www.ebi.ac.uk/pdbsum/4r0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0o ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0o OCA], [https://pdbe.org/4r0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r0o RCSB], [https://www.ebi.ac.uk/pdbsum/4r0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0o ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PLAS_PHOLA PLAS_PHOLA]] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
+[https://www.uniprot.org/uniprot/PLAS_PHOLA PLAS_PHOLA] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 4r0o" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
 == References ==
 <references/>
@@ Line 24: / Line 27: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Phola]]
+[[Category: Phormidium laminosum]]
-[[Category: Cattani, G]]
+[[Category: Cattani G]]
-[[Category: Crowley, P B]]
+[[Category: Crowley PB]]
-[[Category: Vogeley, L]]
+[[Category: Vogeley L]]
-[[Category: Electron transport]]
-[[Category: Pegylation]]

4r0o

From Proteopedia

Current revision

Crystal structure of PEGylated plastocyanin at 4.2 A resolution

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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