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| | <StructureSection load='4q65' size='340' side='right'caption='[[4q65]], [[Resolution|resolution]] 3.40Å' scene=''> | | <StructureSection load='4q65' size='340' side='right'caption='[[4q65]], [[Resolution|resolution]] 3.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4q65]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q65 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4q65]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q65 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dtpD, ybgH, b0709, JW0699 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q65 OCA], [http://pdbe.org/4q65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q65 RCSB], [http://www.ebi.ac.uk/pdbsum/4q65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q65 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q65 OCA], [https://pdbe.org/4q65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q65 RCSB], [https://www.ebi.ac.uk/pdbsum/4q65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q65 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DTPD_ECOLI DTPD_ECOLI]] Probable proton-dependent permease that transports dipeptides (By similarity). | + | [https://www.uniprot.org/uniprot/DTPD_ECOLI DTPD_ECOLI] Probable proton-dependent permease that transports dipeptides (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Liu, M]] | + | [[Category: Liu M]] |
| - | [[Category: Mao, G]] | + | [[Category: Mao G]] |
| - | [[Category: Wang, X]] | + | [[Category: Wang X]] |
| - | [[Category: Zhang, C]] | + | [[Category: Zhang C]] |
| - | [[Category: Zhao, Y]] | + | [[Category: Zhao Y]] |
| - | [[Category: Mfs fold motif some]]
| + | |
| - | [[Category: Peptide transporter]]
| + | |
| - | [[Category: Transport protein]]
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| Structural highlights
Function
DTPD_ECOLI Probable proton-dependent permease that transports dipeptides (By similarity).
Publication Abstract from PubMed
E. coli YbgH belongs to the family of proton-dependent oligopeptide transporters (POTs), a subfamily of the major facilitator superfamily (MFS) of secondary active transporters. Like other MFS transporters, POT proteins switch between two major conformations during substrate transport. Apart from possessing a canonical 12-helix, two-domain transmembrane (TM) core, prokaryotic POT proteins usually have two TM helices inserted between the two domains. Here we determined the crystal structure of YbgH in its inward-facing conformation. Our structure-based functional studies investigated the roles of both the POT signature motif 2 and the inserted interdomain TM helix pair in the stabilization and regulation of the major conformational change in MFS/POT transporters. Furthermore, of all the proton-titratable amino acid residues, Glu21 is the only conserved one (among POTs) located in the central cavity and is critical for in vivo transport. Together, our results support the notion that MFS symporters utilize a transport mechanism based on substrate-protonation coupling.
Crystal Structure of the E. coli Peptide Transporter YbgH.,Zhao Y, Mao G, Liu M, Zhang L, Wang X, Zhang XC Structure. 2014 Aug 5;22(8):1152-60. doi: 10.1016/j.str.2014.06.008. Epub 2014, Jul 24. PMID:25066136[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhao Y, Mao G, Liu M, Zhang L, Wang X, Zhang XC. Crystal Structure of the E. coli Peptide Transporter YbgH. Structure. 2014 Aug 5;22(8):1152-60. doi: 10.1016/j.str.2014.06.008. Epub 2014, Jul 24. PMID:25066136 doi:http://dx.doi.org/10.1016/j.str.2014.06.008
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