2bze

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[[Image:2bze.gif|left|200px]]
 
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==NMR Structure of human RTF1 PLUS3 domain.==
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The line below this paragraph, containing "STRUCTURE_2bze", creates the "Structure Box" on the page.
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<StructureSection load='2bze' size='340' side='right'caption='[[2bze]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BZE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bze OCA], [https://pdbe.org/2bze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bze RCSB], [https://www.ebi.ac.uk/pdbsum/2bze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bze ProSAT]</span></td></tr>
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{{STRUCTURE_2bze| PDB=2bze | SCENE= }}
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</table>
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== Evolutionary Conservation ==
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'''NMR STRUCTURE OF HUMAN RTF1 PLUS3 DOMAIN.'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/2bze_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bze ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The yeast Paf1 complex consists of Paf1, Rtf1, Cdc73, Ctr9, and Leo1 and regulates histone H2B ubiquitination, histone H3 methylation, RNA polymerase II carboxy-terminal domain (CTD) Ser2 phosphorylation, and RNA 3' end processing. We provide structural insight into the Paf1 complex with the NMR structure of the conserved and functionally important Plus3 domain of human Rtf1. A predominantly beta-stranded subdomain displays structural similarity to Dicer/Argonaute PAZ domains and to Tudor domains. We further demonstrate that the highly basic Rtf1 Plus3 domain can interact in vitro with single-stranded DNA via residues on the rim of the beta sheet, reminiscent of siRNA binding by PAZ domains, but did not detect binding to double-stranded DNA or RNA. We discuss the potential role of Rtf1 Plus3 ssDNA binding during transcription elongation.
The yeast Paf1 complex consists of Paf1, Rtf1, Cdc73, Ctr9, and Leo1 and regulates histone H2B ubiquitination, histone H3 methylation, RNA polymerase II carboxy-terminal domain (CTD) Ser2 phosphorylation, and RNA 3' end processing. We provide structural insight into the Paf1 complex with the NMR structure of the conserved and functionally important Plus3 domain of human Rtf1. A predominantly beta-stranded subdomain displays structural similarity to Dicer/Argonaute PAZ domains and to Tudor domains. We further demonstrate that the highly basic Rtf1 Plus3 domain can interact in vitro with single-stranded DNA via residues on the rim of the beta sheet, reminiscent of siRNA binding by PAZ domains, but did not detect binding to double-stranded DNA or RNA. We discuss the potential role of Rtf1 Plus3 ssDNA binding during transcription elongation.
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==About this Structure==
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Structure and DNA binding of the human Rtf1 Plus3 domain.,de Jong RN, Truffault V, Diercks T, Ab E, Daniels MA, Kaptein R, Folkers GE Structure. 2008 Jan;16(1):149-59. PMID:18184592<ref>PMID:18184592</ref>
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2BZE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZE OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure and DNA binding of the human Rtf1 Plus3 domain., de Jong RN, Truffault V, Diercks T, Ab E, Daniels MA, Kaptein R, Folkers GE, Structure. 2008 Jan;16(1):149-59. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18184592 18184592]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 2bze" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Ab, E.]]
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<references/>
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[[Category: Daniels, M A.]]
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__TOC__
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[[Category: Diercks, T.]]
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</StructureSection>
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[[Category: Folkers, G E.]]
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[[Category: Large Structures]]
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[[Category: Jong, R N.De.]]
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[[Category: Ab E]]
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[[Category: Kaptein, R.]]
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[[Category: Daniels MA]]
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[[Category: Spine, Structural Proteomics in Europe.]]
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[[Category: De Jong RN]]
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[[Category: Truffault, V.]]
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[[Category: Diercks T]]
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[[Category: Cdc73]]
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[[Category: Folkers GE]]
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[[Category: Ctr9]]
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[[Category: Kaptein R]]
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[[Category: Elongation]]
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[[Category: Truffault V]]
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[[Category: H2b ubiquitination]]
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[[Category: Histone h3 methylation]]
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[[Category: Human rtf1 plus3 domain]]
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[[Category: Leo1]]
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[[Category: Paf1 complex]]
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[[Category: Plus3 domain]]
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[[Category: Spine]]
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[[Category: Structural genomic]]
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[[Category: Structural proteomics in europe]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:03:26 2008''
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Current revision

NMR Structure of human RTF1 PLUS3 domain.

PDB ID 2bze

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