1mb0

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[[Image:1mb0.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF THE RESPONSE REGULATOR DIVK AT PH 8.0 IN COMPLEX WITH MN2+==
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The line below this paragraph, containing "STRUCTURE_1mb0", creates the "Structure Box" on the page.
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<StructureSection load='1mb0' size='340' side='right'caption='[[1mb0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mb0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MB0 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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{{STRUCTURE_1mb0| PDB=1mb0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mb0 OCA], [https://pdbe.org/1mb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mb0 RCSB], [https://www.ebi.ac.uk/pdbsum/1mb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mb0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9A5I4_CAUVC Q9A5I4_CAUVC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mb/1mb0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mb0 ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF THE RESPONSE REGULATOR DIVK AT PH 8.0 IN COMPLEX WITH MN2+'''
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==See Also==
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*[[Response regulator 3D structure|Response regulator 3D structure]]
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__TOC__
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==Overview==
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</StructureSection>
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DivK is an essential response regulator in the Gram-negative bacterium Caulobacter crescentus and functions in a complex phosphorelay system that precisely controls the sequence of developmental events during the cell division cycle. Structure determinations of this single domain response regulator at different pH values demonstrated that the five-stranded alpha/beta fold of the DivK protein is fully defined only at acidic pH. The crystal structures of the apoprotein and of metal-bound DivK complexes at higher pH values revealed a synergistic pH- and cation binding-induced flexibility of the beta4-alpha4 loop and of the alpha4 helix. This motion increases the solvent accessibility of the single cysteine residue in the protein. Solution state studies demonstrated a 200-fold pH-dependent increase in the affinity of manganese for the protein between pH 6.0 and 8.5 that seems to involve deprotonation of an acido-basic couple. Taken together, these results suggest that flexibility of critical regions of the protein, ionization of the cysteine 99 residue and improved K(D) values for the catalytic metal ion are coupled events. We propose that the molecular events observed in the isolated protein may be required for DivK activation and that they may be achieved in vivo through the specific protein-protein interactions between the response regulator and its cognate kinases.
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==About this Structure==
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1MB0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MB0 OCA].
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==Reference==
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Crystallographic and biochemical studies of DivK reveal novel features of an essential response regulator in Caulobacter crescentus., Guillet V, Ohta N, Cabantous S, Newton A, Samama JP, J Biol Chem. 2002 Nov 1;277(44):42003-10. Epub 2002 Aug 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12176983 12176983]
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[[Category: Caulobacter vibrioides]]
[[Category: Caulobacter vibrioides]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cabantous, S.]]
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[[Category: Cabantous S]]
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[[Category: Guillet, V.]]
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[[Category: Guillet V]]
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[[Category: Newton, A.]]
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[[Category: Newton A]]
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[[Category: Ohta, N.]]
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[[Category: Ohta N]]
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[[Category: SPINE, Structural Proteomics in Europe.]]
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[[Category: Samama J-P]]
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[[Category: Samama, J-P.]]
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[[Category: Response regulator]]
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[[Category: Signal transduction protein]]
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[[Category: Spine]]
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[[Category: Structural genomic]]
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[[Category: Structural proteomics in europe]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:07:31 2008''
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Current revision

CRYSTAL STRUCTURE OF THE RESPONSE REGULATOR DIVK AT PH 8.0 IN COMPLEX WITH MN2+

PDB ID 1mb0

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