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6jd1

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==Cryo-EM Structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) in complex with Mg2+, NADH, and CPD at pH7.5==
==Cryo-EM Structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) in complex with Mg2+, NADH, and CPD at pH7.5==
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<StructureSection load='6jd1' size='340' side='right'caption='[[6jd1]], [[Resolution|resolution]] 3.38&Aring;' scene=''>
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<SX load='6jd1' size='340' side='right' viewer='molstar' caption='[[6jd1]], [[Resolution|resolution]] 3.38&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6jd1]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JD1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JD1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6jd1]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JD1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JD1 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=9TY:cyclopropane-1,1-dicarboxylic+acid'>9TY</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.38&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ilvC2, ilvC-2, SSO1322 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9TY:cyclopropane-1,1-dicarboxylic+acid'>9TY</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ketol-acid_reductoisomerase Ketol-acid reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.86 1.1.1.86] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jd1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jd1 OCA], [https://pdbe.org/6jd1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jd1 RCSB], [https://www.ebi.ac.uk/pdbsum/6jd1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jd1 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jd1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jd1 OCA], [http://pdbe.org/6jd1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jd1 RCSB], [http://www.ebi.ac.uk/pdbsum/6jd1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jd1 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/ILVC2_SACS2 ILVC2_SACS2]
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While cryo-EM is revolutionizing structural biology, its impact on enzymology is yet to be fully demonstrated. The ketol-acid reductoisomerase (KARI) catalyzes conversion of (2 S)-acetolactate or (2 S)-aceto-2-hydroxybutyrate to 2,3-dihydroxy-3-alkylbutyrate. We found that KARI from archaea Sulfolobus solfataricus (Sso-KARI) is unusual in being a dodecamer, bispecific to NADH and NADPH, and losing activity above pH 7.8. While crystals were obtainable only at pH 8.5, cryo-EM structures were solved at pH 7.5 and 8.5 for Sso-KARI:2Mg(2+). The results showed that the distances of the two catalytic Mg(2+) ions are lengthened in both structures at pH 8.5. We next solved cryo-EM structures of two Sso-KARI complexes, with NADH+inhibitor and NADPH+inhibitor at pH 7.5, which indicate that the bispecificity can be attributed to a unique asparagine at the cofactor binding loop. Unexpectedly, Sso-KARI also differs from other KARI enzymes in lacking "induced-fit", reflecting structural rigidity. Thus, cryo-EM is powerful for structural and mechanistic enzymology.
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Use of Cryo-EM To Uncover Structural Bases of pH Effect and Cofactor Bispecificity of Ketol-Acid Reductoisomerase.,Chen CY, Chang YC, Lin BL, Lin KF, Huang CH, Hsieh DL, Ko TP, Tsai MD J Am Chem Soc. 2019 Apr 2. doi: 10.1021/jacs.9b01354. PMID:30921515<ref>PMID:30921515</ref>
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==See Also==
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*[[Ketol-acid reductoisomerase 3D structures|Ketol-acid reductoisomerase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6jd1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Ketol-acid reductoisomerase]]
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Sacs2]]
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[[Category: Saccharolobus solfataricus P2]]
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[[Category: Chang, Y C]]
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[[Category: Chang YC]]
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[[Category: Chen, C Y]]
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[[Category: Chen CY]]
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[[Category: Hsieh, D L]]
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[[Category: Hsieh DL]]
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[[Category: Huang, C H]]
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[[Category: Huang CH]]
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[[Category: Ko, T P]]
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[[Category: Ko TP]]
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[[Category: Lin, B L]]
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[[Category: Lin BL]]
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[[Category: Lin, K F]]
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[[Category: Lin KF]]
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[[Category: Tsai, M D]]
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[[Category: Tsai MD]]
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[[Category: Bi-specific]]
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[[Category: Dodecamer]]
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[[Category: Isomerase]]
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[[Category: Knotted protein]]
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[[Category: Magnesium-dependent]]
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[[Category: Reductoisomerase]]
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[[Category: Thermostable]]
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Current revision

Cryo-EM Structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) in complex with Mg2+, NADH, and CPD at pH7.5

6jd1, resolution 3.38Å

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