|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6nbu' size='340' side='right'caption='[[6nbu]], [[Resolution|resolution]] 2.75Å' scene=''> | | <StructureSection load='6nbu' size='340' side='right'caption='[[6nbu]], [[Resolution|resolution]] 2.75Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6nbu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staeq Staeq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NBU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nbu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis_RP62A Staphylococcus epidermidis RP62A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NBU FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SERP2460 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176279 STAEQ])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nbu OCA], [http://pdbe.org/6nbu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nbu RCSB], [http://www.ebi.ac.uk/pdbsum/6nbu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nbu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nbu OCA], [https://pdbe.org/6nbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nbu RCSB], [https://www.ebi.ac.uk/pdbsum/6nbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nbu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q5HK90_STAEQ Q5HK90_STAEQ] |
| - | Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated Cas proteins provide an immune-like response in many prokaryotes against extraneous nucleic acids. CRISPR-Cas systems are classified into different classes and types. Class 1 CRISPR-Cas systems form multi-protein effector complexes that includes a guide RNA (crRNA) used to identify the target for destruction. Here we present crystal structures of Staphylococcus epidermidis Type III-A CRISPR subunits Csm2 and Csm3 and a 5.2 A resolution single-particle cryo-electron microscopy (cryo-EM) reconstruction of an in vivo assembled effector subcomplex including the crRNA. The structures help to clarify the quaternary architecture of Type III-A effector complexes, and provide details on crRNA binding, target RNA binding and cleavage, and intermolecular interactions essential for effector complex assembly. The structures allow a better understanding of the organization of Type III-A CRISPR effector complexes as well as highlighting the overall similarities and differences with other Class 1 effector complexes.
| + | |
| - | | + | |
| - | Structural organization of a Type III-A CRISPR effector subcomplex determined by X-ray crystallography and cryo-EM.,Dorsey BW, Huang L, Mondragon A Nucleic Acids Res. 2019 Feb 13. pii: 5310821. doi: 10.1093/nar/gkz079. PMID:30759237<ref>PMID:30759237</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6nbu" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Staeq]] | + | [[Category: Staphylococcus epidermidis RP62A]] |
| - | [[Category: Dorsey, B W]] | + | [[Category: Dorsey BW]] |
| - | [[Category: Huang, L]] | + | [[Category: Huang L]] |
| - | [[Category: Mondragon, A]] | + | [[Category: Mondragon A]] |
| - | [[Category: Crispr]]
| + | |
| - | [[Category: Structural protein]]
| + | |
