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| | <StructureSection load='4r83' size='340' side='right'caption='[[4r83]], [[Resolution|resolution]] 1.93Å' scene=''> | | <StructureSection load='4r83' size='340' side='right'caption='[[4r83]], [[Resolution|resolution]] 1.93Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4r83]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33539 Atcc 33539]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R83 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R83 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4r83]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Photobacterium_damselae Photobacterium damselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R83 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R83 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4r84|4r84]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bst ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38293 ATCC 33539])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r83 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r83 OCA], [https://pdbe.org/4r83 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r83 RCSB], [https://www.ebi.ac.uk/pdbsum/4r83 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r83 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactoside_alpha-2,6-sialyltransferase Beta-galactoside alpha-2,6-sialyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.99.1 2.4.99.1] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r83 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r83 OCA], [http://pdbe.org/4r83 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r83 RCSB], [http://www.ebi.ac.uk/pdbsum/4r83 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r83 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O66375_9GAMM O66375_9GAMM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Sialyltransferase|Sialyltransferase]] | + | *[[Sialyltransferase 3D structures|Sialyltransferase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 33539]] | |
| - | [[Category: Beta-galactoside alpha-2,6-sialyltransferase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chen, X]] | + | [[Category: Photobacterium damselae]] |
| - | [[Category: Fisher, A J]] | + | [[Category: Chen X]] |
| - | [[Category: Huynh, N]] | + | [[Category: Fisher AJ]] |
| - | [[Category: Li, Y]] | + | [[Category: Huynh N]] |
| - | [[Category: Glycosyltransferase gt-b structural group]]
| + | [[Category: Li Y]] |
| - | [[Category: Rossmann fold]]
| + | |
| - | [[Category: Transferase]]
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| Structural highlights
Function
O66375_9GAMM
Publication Abstract from PubMed
Sialyltransferase structures fall into either GT-A or GT-B glycosyltransferase fold. Some sialyltransferases from the Photobacterium genus have been shown to contain an additional N-terminal immunoglobulin (Ig)-like domain. Photobacterium damselae alpha2-6-sialyltransferase has been used efficiently in enzymatic and chemoenzymatic synthesis of alpha2-6-linked sialosides. Here we report three crystal structures of this enzyme. Two structures with and without a donor substrate analog CMP-3F(a)Neu5Ac contain an immunoglobulin (Ig)-like domain and adopt the GT-B sialyltransferase fold. The binary structure reveals a non-productive pre-Michaelis complex, which are caused by crystal lattice contacts that prevent the large conformational changes. The third structure lacks the Ig-domain. Comparison of the three structures reveals small inherent flexibility between the two Rossmann-like domains of the GT-B fold.
Crystal structures of sialyltransferase from Photobacterium damselae.,Huynh N, Li Y, Yu H, Huang S, Lau K, Chen X, Fisher AJ FEBS Lett. 2014 Dec 20;588(24):4720-9. doi: 10.1016/j.febslet.2014.11.003. Epub, 2014 Nov 15. PMID:25451227[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Huynh N, Li Y, Yu H, Huang S, Lau K, Chen X, Fisher AJ. Crystal structures of sialyltransferase from Photobacterium damselae. FEBS Lett. 2014 Dec 20;588(24):4720-9. doi: 10.1016/j.febslet.2014.11.003. Epub, 2014 Nov 15. PMID:25451227 doi:http://dx.doi.org/10.1016/j.febslet.2014.11.003
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