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| | <StructureSection load='4r8r' size='340' side='right'caption='[[4r8r]], [[Resolution|resolution]] 1.46Å' scene=''> | | <StructureSection load='4r8r' size='340' side='right'caption='[[4r8r]], [[Resolution|resolution]] 1.46Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4r8r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R8R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R8R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4r8r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R8R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R8R FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4r8s|4r8s]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.46Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r8r OCA], [http://pdbe.org/4r8r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r8r RCSB], [http://www.ebi.ac.uk/pdbsum/4r8r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r8r ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r8r OCA], [https://pdbe.org/4r8r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r8r RCSB], [https://www.ebi.ac.uk/pdbsum/4r8r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r8r ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/C1KBQ3_9FLAV C1KBQ3_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774] | + | [https://www.uniprot.org/uniprot/C1KBQ3_9FLAV C1KBQ3_9FLAV] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Nonstructural protein|Nonstructural protein]] | + | *[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | [[Category: Dengue virus 3]] | | [[Category: Dengue virus 3]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Noble, C G]] | + | [[Category: Noble CG]] |
| - | [[Category: 2'o methyltransferase]]
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| - | [[Category: N7 methyltransferase]]
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| - | [[Category: Transferase]]
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| Structural highlights
Function
C1KBQ3_9FLAV Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774]
Publication Abstract from PubMed
Flavivirus methyltransferase is a genetically-validated antiviral target. Crystal structures of almost all available flavivirus methyltransferases contain S-adenosyl-L-methionine (SAM), the methyl donor molecule that co-purifies with the enzymes. This raises a possibility that SAM is an integral structural component required for the folding of dengue virus (DENV) methyltransferase. Here we exclude this possibility by solving the crystal structure of DENV methyltransferase without SAM. The SAM ligand was removed from the enzyme through a urea-mediated denaturation-and-renaturation protocol. The crystal structure of the SAM-depleted enzyme exhibits a vacant SAM-binding pocket, with a conformation identical to that of the SAM-enzyme co-crystal structure. Functionally, equivalent enzymatic activities (N-7 methylation, 2'-O methylation, and GMP-enzyme complex formation) were detected for the SAM-depleted and SAM-containing recombinant proteins. These results clearly indicate that the SAM molecule is not an essential component for the correct folding of DENV methyltransferase. Furthermore, the results imply a potential antiviral approach to search for inhibitors that can bind to the SAM-binding pocket and compete against SAM binding. To demonstrate this potential, we have soaked crystals of DENV methyltransferase without a bound SAM with the natural product Sinefungin and show that preformed crystals are capable of binding ligands in this pocket.
Crystal structure of dengue virus methyltransferase without S-adenosyl-L-methionine.,Noble CG, Li SH, Dong H, Chew SH, Shi PY Antiviral Res. 2014 Sep 19;111C:78-81. doi: 10.1016/j.antiviral.2014.09.003. PMID:25241250[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Noble CG, Li SH, Dong H, Chew SH, Shi PY. Crystal structure of dengue virus methyltransferase without S-adenosyl-L-methionine. Antiviral Res. 2014 Sep 19;111C:78-81. doi: 10.1016/j.antiviral.2014.09.003. PMID:25241250 doi:http://dx.doi.org/10.1016/j.antiviral.2014.09.003
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